<article>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#article10_01_26_165254</id>
	<title>Open Source Software Meets Do-It-Yourself Biology</title>
	<author>Soulskill</author>
	<datestamp>1264527000000</datestamp>
	<htmltext>destinyland writes <i>"This article profiles a growing movement &mdash; DIY biology &mdash; that's made possible in part by open source tools. Using programs like BioPerl and BioPython, <a href="http://hplusmagazine.com/articles/bio/diy-bio-growing-movement-takes-aging">DIY biologists write their own code (computer and genetic)</a>, designing their own biological systems and altering the genome. A protein-folding simulator, Folding@home, is now the most powerful distributed computing cluster in the world, and as the movement evolves, cooperatives are also springing up where hobbyists pool resources and create 'hacker spaces' to reduce costs and share knowledge.  'As the shift to open source software continues, computational biology will become even more accessible, and even more powerful,' this article argues &mdash; while intellectual property and other bureaucracies continue to hobble traditional forms of research."</i></htmltext>
<tokenext>destinyland writes " This article profiles a growing movement    DIY biology    that 's made possible in part by open source tools .
Using programs like BioPerl and BioPython , DIY biologists write their own code ( computer and genetic ) , designing their own biological systems and altering the genome .
A protein-folding simulator , Folding @ home , is now the most powerful distributed computing cluster in the world , and as the movement evolves , cooperatives are also springing up where hobbyists pool resources and create 'hacker spaces ' to reduce costs and share knowledge .
'As the shift to open source software continues , computational biology will become even more accessible , and even more powerful, ' this article argues    while intellectual property and other bureaucracies continue to hobble traditional forms of research .
"</tokentext>
<sentencetext>destinyland writes "This article profiles a growing movement — DIY biology — that's made possible in part by open source tools.
Using programs like BioPerl and BioPython, DIY biologists write their own code (computer and genetic), designing their own biological systems and altering the genome.
A protein-folding simulator, Folding@home, is now the most powerful distributed computing cluster in the world, and as the movement evolves, cooperatives are also springing up where hobbyists pool resources and create 'hacker spaces' to reduce costs and share knowledge.
'As the shift to open source software continues, computational biology will become even more accessible, and even more powerful,' this article argues — while intellectual property and other bureaucracies continue to hobble traditional forms of research.
"</sentencetext>
</article>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30907274</id>
	<title>Re:Uhhh...</title>
	<author>svtdragon</author>
	<datestamp>1264532460000</datestamp>
	<modclass>None</modclass>
	<modscore>1</modscore>
	<htmltext>Useful as it sounds, I think DIY bioweapons could be a *small* concern.  Especially if they come with instructions better than Ikea's.</htmltext>
<tokenext>Useful as it sounds , I think DIY bioweapons could be a * small * concern .
Especially if they come with instructions better than Ikea 's .</tokentext>
<sentencetext>Useful as it sounds, I think DIY bioweapons could be a *small* concern.
Especially if they come with instructions better than Ikea's.</sentencetext>
	<parent>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30906858</parent>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30911046</id>
	<title>Re:IP is not hobbling traditional research</title>
	<author>martin-boundary</author>
	<datestamp>1264505520000</datestamp>
	<modclass>Insightful</modclass>
	<modscore>2</modscore>
	<htmltext>Your linked paper is a report by a bunch of non-lawyers asking working scientists whether they think their work is adversely affected by IP law, and you consider that
useful why?
<p>
We already know that most people break IP laws all the time, often without realizing it. Would you also quote a paper that claims the copyright threat is overblown, because the vast majority of music downloaders self-report that they aren't being sued?
</p><p>
The real problem is that the IP laws exist in the first place: they are a <a href="http://en.wikipedia.org/wiki/Sword\_of\_damocles" title="wikipedia.org">Sword of Damocles</a> [wikipedia.org] upon researchers, whether they look up or not.</p></div>
	</htmltext>
<tokenext>Your linked paper is a report by a bunch of non-lawyers asking working scientists whether they think their work is adversely affected by IP law , and you consider that useful why ?
We already know that most people break IP laws all the time , often without realizing it .
Would you also quote a paper that claims the copyright threat is overblown , because the vast majority of music downloaders self-report that they are n't being sued ?
The real problem is that the IP laws exist in the first place : they are a Sword of Damocles [ wikipedia.org ] upon researchers , whether they look up or not .</tokentext>
<sentencetext>Your linked paper is a report by a bunch of non-lawyers asking working scientists whether they think their work is adversely affected by IP law, and you consider that
useful why?
We already know that most people break IP laws all the time, often without realizing it.
Would you also quote a paper that claims the copyright threat is overblown, because the vast majority of music downloaders self-report that they aren't being sued?
The real problem is that the IP laws exist in the first place: they are a Sword of Damocles [wikipedia.org] upon researchers, whether they look up or not.
	</sentencetext>
	<parent>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30907406</parent>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30914034</id>
	<title>Viva la resistance</title>
	<author>morty\_vikka</author>
	<datestamp>1264532040000</datestamp>
	<modclass>None</modclass>
	<modscore>1</modscore>
	<htmltext>Um, cloning genes into plasmids and propagating them in E.coli so that you can manipulate (mutate) the sequence invariably requires some kind of antibiotic selection. Letting people muck around in their garages with plasmids conferring antibiotics resistance in bugs that have the potential to become resident in your intestines and persist indefinitely in the environment is a horrendously bad idea. <br> <br>

It's why all labs that do this stuff routinely are required to adhere to physical containment guidelines.  <br> <br>

Pretty fucking scary indeed.</htmltext>
<tokenext>Um , cloning genes into plasmids and propagating them in E.coli so that you can manipulate ( mutate ) the sequence invariably requires some kind of antibiotic selection .
Letting people muck around in their garages with plasmids conferring antibiotics resistance in bugs that have the potential to become resident in your intestines and persist indefinitely in the environment is a horrendously bad idea .
It 's why all labs that do this stuff routinely are required to adhere to physical containment guidelines .
Pretty fucking scary indeed .</tokentext>
<sentencetext>Um, cloning genes into plasmids and propagating them in E.coli so that you can manipulate (mutate) the sequence invariably requires some kind of antibiotic selection.
Letting people muck around in their garages with plasmids conferring antibiotics resistance in bugs that have the potential to become resident in your intestines and persist indefinitely in the environment is a horrendously bad idea.
It's why all labs that do this stuff routinely are required to adhere to physical containment guidelines.
Pretty fucking scary indeed.</sentencetext>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30906914</id>
	<title>This only makes sense.</title>
	<author>Anonymous</author>
	<datestamp>1264531020000</datestamp>
	<modclass>Troll</modclass>
	<modscore>-1</modscore>
	<htmltext>The OSS crowd is looking for a cure for AIDS because they all eat the shit out of the asses of other men and take cocks up their shit chute.<br> <br>Fucking dirty bird faggots.</htmltext>
<tokenext>The OSS crowd is looking for a cure for AIDS because they all eat the shit out of the asses of other men and take cocks up their shit chute .
Fucking dirty bird faggots .</tokentext>
<sentencetext>The OSS crowd is looking for a cure for AIDS because they all eat the shit out of the asses of other men and take cocks up their shit chute.
Fucking dirty bird faggots.</sentencetext>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30907050</id>
	<title>Re:Uhhh...</title>
	<author>fuzzyfuzzyfungus</author>
	<datestamp>1264531620000</datestamp>
	<modclass>Insightful</modclass>
	<modscore>4</modscore>
	<htmltext>On the plus side, doing dangerous things that are more dangerous to others than to yourself is substantially harder than just doing dangerous things.<br> <br>

Until we get to the point where you can just buy a programmable matter synthesizer with a voice interface that will accept the command "50grams aerosolized anthrax, weapons grade" the only real danger of DIY Biology will be a few scientist wannabees ending up in the ER on a stiff antibiotic drip after spilling the wrong bacterial culture on themselves.<br> <br>

DIY Bio is novel, and sciencey, which makes it OOH Scary; but, if you just want to hurt some people, good old-fashioned all-american firearms are <i>way</i> easier, cheaper, and substantially more refined.</htmltext>
<tokenext>On the plus side , doing dangerous things that are more dangerous to others than to yourself is substantially harder than just doing dangerous things .
Until we get to the point where you can just buy a programmable matter synthesizer with a voice interface that will accept the command " 50grams aerosolized anthrax , weapons grade " the only real danger of DIY Biology will be a few scientist wannabees ending up in the ER on a stiff antibiotic drip after spilling the wrong bacterial culture on themselves .
DIY Bio is novel , and sciencey , which makes it OOH Scary ; but , if you just want to hurt some people , good old-fashioned all-american firearms are way easier , cheaper , and substantially more refined .</tokentext>
<sentencetext>On the plus side, doing dangerous things that are more dangerous to others than to yourself is substantially harder than just doing dangerous things.
Until we get to the point where you can just buy a programmable matter synthesizer with a voice interface that will accept the command "50grams aerosolized anthrax, weapons grade" the only real danger of DIY Biology will be a few scientist wannabees ending up in the ER on a stiff antibiotic drip after spilling the wrong bacterial culture on themselves.
DIY Bio is novel, and sciencey, which makes it OOH Scary; but, if you just want to hurt some people, good old-fashioned all-american firearms are way easier, cheaper, and substantially more refined.</sentencetext>
	<parent>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30906858</parent>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30909208</id>
	<title>Andrew Ryan Already Tried This</title>
	<author>BJ\_Covert\_Action</author>
	<datestamp>1264497480000</datestamp>
	<modclass>None</modclass>
	<modscore>1</modscore>
	<htmltext>Don't you guys remember the underwater city of Rapture? It became easy and accessible to splice one's own gene sequence and all hell broke loose down there. What did you think those splicers were doing? Heroine? Mark my words, this will start with harmless science projects but soon the populous will be killing little girls for ADAM and shooting lighting at each other out of their hands. Hell's come the surface!</htmltext>
<tokenext>Do n't you guys remember the underwater city of Rapture ?
It became easy and accessible to splice one 's own gene sequence and all hell broke loose down there .
What did you think those splicers were doing ?
Heroine ? Mark my words , this will start with harmless science projects but soon the populous will be killing little girls for ADAM and shooting lighting at each other out of their hands .
Hell 's come the surface !</tokentext>
<sentencetext>Don't you guys remember the underwater city of Rapture?
It became easy and accessible to splice one's own gene sequence and all hell broke loose down there.
What did you think those splicers were doing?
Heroine? Mark my words, this will start with harmless science projects but soon the populous will be killing little girls for ADAM and shooting lighting at each other out of their hands.
Hell's come the surface!</sentencetext>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30908346</id>
	<title>Not a surprise</title>
	<author>Scribbler'sEmporium</author>
	<datestamp>1264536780000</datestamp>
	<modclass>None</modclass>
	<modscore>1</modscore>
	<htmltext>Biology has not traditionally been computationally intensive, but that has changed in the last 20 years. Computer modelling of ecology, disease epidemiology, and cellular processes has been tremendously helpful. With the age of the genome merging into the age of the proteome the trend is only going to grow. Biology is or will shortly be as computationally intensive as physics. Witness the explosion of Bioinformatics departments in Universities in the last 10 years.

The main trouble is that most of the problems in biology (finding patterns in nucleotide sequences for example) are not interesting problems for computer scientists. The computational techniques, for the most part have been worked out and are just a matter of number crunching. The trick now is to employ them on the mountain of data generated by the biologists.

It doesn't surpise me that biologists are learning to do their own coding, or at least learning enough of the lingo to go talk to the local programmers.</htmltext>
<tokenext>Biology has not traditionally been computationally intensive , but that has changed in the last 20 years .
Computer modelling of ecology , disease epidemiology , and cellular processes has been tremendously helpful .
With the age of the genome merging into the age of the proteome the trend is only going to grow .
Biology is or will shortly be as computationally intensive as physics .
Witness the explosion of Bioinformatics departments in Universities in the last 10 years .
The main trouble is that most of the problems in biology ( finding patterns in nucleotide sequences for example ) are not interesting problems for computer scientists .
The computational techniques , for the most part have been worked out and are just a matter of number crunching .
The trick now is to employ them on the mountain of data generated by the biologists .
It does n't surpise me that biologists are learning to do their own coding , or at least learning enough of the lingo to go talk to the local programmers .</tokentext>
<sentencetext>Biology has not traditionally been computationally intensive, but that has changed in the last 20 years.
Computer modelling of ecology, disease epidemiology, and cellular processes has been tremendously helpful.
With the age of the genome merging into the age of the proteome the trend is only going to grow.
Biology is or will shortly be as computationally intensive as physics.
Witness the explosion of Bioinformatics departments in Universities in the last 10 years.
The main trouble is that most of the problems in biology (finding patterns in nucleotide sequences for example) are not interesting problems for computer scientists.
The computational techniques, for the most part have been worked out and are just a matter of number crunching.
The trick now is to employ them on the mountain of data generated by the biologists.
It doesn't surpise me that biologists are learning to do their own coding, or at least learning enough of the lingo to go talk to the local programmers.</sentencetext>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30907670</id>
	<title>Well, this is a new wrinke on the "bug" concept</title>
	<author>gestalt\_n\_pepper</author>
	<datestamp>1264534320000</datestamp>
	<modclass>None</modclass>
	<modscore>1</modscore>
	<htmltext><p>"Sir, the new beetle you were working on? It crashed. Shall I reboot it?"</p></htmltext>
<tokenext>" Sir , the new beetle you were working on ?
It crashed .
Shall I reboot it ?
"</tokentext>
<sentencetext>"Sir, the new beetle you were working on?
It crashed.
Shall I reboot it?
"</sentencetext>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30907108</id>
	<title>Hurray great article!</title>
	<author>Vamman</author>
	<datestamp>1264531800000</datestamp>
	<modclass>Funny</modclass>
	<modscore>2</modscore>
	<htmltext>Awesome article. Our team has embraced the use of the R Stats package in our environmental assessment tool. We were sick of the COM object library to connect modern<nobr> <wbr></nobr>.NET tools to our tool so I decided to build a<nobr> <wbr></nobr>.NET wrapper for R. Still in early development but it works for us. We decided to release it under GPL for everyone to use.  I think the title of article could read something like "Biologists take programming into their own hands" which is what I was forced to do during my MSc. and now once again in my position at U of S I find myself hanging out with the computer scientists a little bit too often.</htmltext>
<tokenext>Awesome article .
Our team has embraced the use of the R Stats package in our environmental assessment tool .
We were sick of the COM object library to connect modern .NET tools to our tool so I decided to build a .NET wrapper for R. Still in early development but it works for us .
We decided to release it under GPL for everyone to use .
I think the title of article could read something like " Biologists take programming into their own hands " which is what I was forced to do during my MSc .
and now once again in my position at U of S I find myself hanging out with the computer scientists a little bit too often .</tokentext>
<sentencetext>Awesome article.
Our team has embraced the use of the R Stats package in our environmental assessment tool.
We were sick of the COM object library to connect modern .NET tools to our tool so I decided to build a .NET wrapper for R. Still in early development but it works for us.
We decided to release it under GPL for everyone to use.
I think the title of article could read something like "Biologists take programming into their own hands" which is what I was forced to do during my MSc.
and now once again in my position at U of S I find myself hanging out with the computer scientists a little bit too often.</sentencetext>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30907930</id>
	<title>Re:Uhhh...</title>
	<author>Anonymous</author>
	<datestamp>1264535220000</datestamp>
	<modclass>None</modclass>
	<modscore>0</modscore>
	<htmltext><p><div class="quote"><p>On the plus side, doing dangerous things that are more dangerous to others than to yourself is substantially harder than just doing dangerous things.</p></div><p>Yes, because what nuts would be suicidal enough to intentionally hurt themselves in the act of hurting others?</p></div>
	</htmltext>
<tokenext>On the plus side , doing dangerous things that are more dangerous to others than to yourself is substantially harder than just doing dangerous things.Yes , because what nuts would be suicidal enough to intentionally hurt themselves in the act of hurting others ?</tokentext>
<sentencetext>On the plus side, doing dangerous things that are more dangerous to others than to yourself is substantially harder than just doing dangerous things.Yes, because what nuts would be suicidal enough to intentionally hurt themselves in the act of hurting others?
	</sentencetext>
	<parent>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30907050</parent>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30908560</id>
	<title>Re:Any progress?</title>
	<author>Explodicle</author>
	<datestamp>1264537500000</datestamp>
	<modclass>Interestin</modclass>
	<modscore>3</modscore>
	<htmltext><p>I'm a mechanical engineer who uses finite element analysis every day. These days are numbered. Every year something new comes out that makes it even easier and more idiot-proof, heading towards the point where really anyone COULD do it. Red = "breaks here". "Would you like to use the Analysis Assistant?"</p><p>The distinction between the expert and the automated amateur is diminishing. Remember when you needed to know HTML to have a web page? It's only now getting started with DIY biology, but just wait... the progress since last time might not be obvious, but it's happening.</p></htmltext>
<tokenext>I 'm a mechanical engineer who uses finite element analysis every day .
These days are numbered .
Every year something new comes out that makes it even easier and more idiot-proof , heading towards the point where really anyone COULD do it .
Red = " breaks here " .
" Would you like to use the Analysis Assistant ?
" The distinction between the expert and the automated amateur is diminishing .
Remember when you needed to know HTML to have a web page ?
It 's only now getting started with DIY biology , but just wait... the progress since last time might not be obvious , but it 's happening .</tokentext>
<sentencetext>I'm a mechanical engineer who uses finite element analysis every day.
These days are numbered.
Every year something new comes out that makes it even easier and more idiot-proof, heading towards the point where really anyone COULD do it.
Red = "breaks here".
"Would you like to use the Analysis Assistant?
"The distinction between the expert and the automated amateur is diminishing.
Remember when you needed to know HTML to have a web page?
It's only now getting started with DIY biology, but just wait... the progress since last time might not be obvious, but it's happening.</sentencetext>
	<parent>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30907092</parent>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30907150</id>
	<title>Re:Uhhh...</title>
	<author>Hatta</author>
	<datestamp>1264531980000</datestamp>
	<modclass>Funny</modclass>
	<modscore>2</modscore>
	<htmltext><p>"Put tab A into slot B" is really all you need to know anyway.</p></htmltext>
<tokenext>" Put tab A into slot B " is really all you need to know anyway .</tokentext>
<sentencetext>"Put tab A into slot B" is really all you need to know anyway.</sentencetext>
	<parent>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30906858</parent>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30907616</id>
	<title>You know what they really want</title>
	<author>SnarfQuest</author>
	<datestamp>1264534020000</datestamp>
	<modclass>None</modclass>
	<modscore>1</modscore>
	<htmltext><p>Those geeky scientists really only want one thing: Cat Girls!</p><p>Girls with cat ears, and a tail. Just like in the manga!</p></htmltext>
<tokenext>Those geeky scientists really only want one thing : Cat Girls ! Girls with cat ears , and a tail .
Just like in the manga !</tokentext>
<sentencetext>Those geeky scientists really only want one thing: Cat Girls!Girls with cat ears, and a tail.
Just like in the manga!</sentencetext>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30910394</id>
	<title>Re:Uhhh...</title>
	<author>pydev</author>
	<datestamp>1264502520000</datestamp>
	<modclass>None</modclass>
	<modscore>1</modscore>
	<htmltext><p><i>DIY Bio is novel, and sciencey, which makes it OOH Scary</i></p><p>If only that were the case; sadly, it isn't.  DIY biology is extremely dangerous in a way that no other technology to date has been.</p></htmltext>
<tokenext>DIY Bio is novel , and sciencey , which makes it OOH ScaryIf only that were the case ; sadly , it is n't .
DIY biology is extremely dangerous in a way that no other technology to date has been .</tokentext>
<sentencetext>DIY Bio is novel, and sciencey, which makes it OOH ScaryIf only that were the case; sadly, it isn't.
DIY biology is extremely dangerous in a way that no other technology to date has been.</sentencetext>
	<parent>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30907050</parent>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30907998</id>
	<title>Re:Uhhh...</title>
	<author>Anonymous</author>
	<datestamp>1264535520000</datestamp>
	<modclass>None</modclass>
	<modscore>0</modscore>
	<htmltext><p>Yay, no more school shootings.  Now angry kids will just make novel time bomb viruses. Oh wait, that was Oryx and Crake.</p></htmltext>
<tokenext>Yay , no more school shootings .
Now angry kids will just make novel time bomb viruses .
Oh wait , that was Oryx and Crake .</tokentext>
<sentencetext>Yay, no more school shootings.
Now angry kids will just make novel time bomb viruses.
Oh wait, that was Oryx and Crake.</sentencetext>
	<parent>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30906858</parent>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30909114</id>
	<title>NCBI's GenBank, PubMed</title>
	<author>bzdyelnik</author>
	<datestamp>1264497060000</datestamp>
	<modclass>Informativ</modclass>
	<modscore>2</modscore>
	<htmltext>more data warehouses:

<a href="http://www.ncbi.nlm.nih.gov/pubmed" title="nih.gov" rel="nofollow">http://www.ncbi.nlm.nih.gov/pubmed</a> [nih.gov]
<a href="http://www.ncbi.nlm.nih.gov/pubmed/" title="nih.gov" rel="nofollow">http://www.ncbi.nlm.nih.gov/pubmed/</a> [nih.gov]</htmltext>
<tokenext>more data warehouses : http : //www.ncbi.nlm.nih.gov/pubmed [ nih.gov ] http : //www.ncbi.nlm.nih.gov/pubmed/ [ nih.gov ]</tokentext>
<sentencetext>more data warehouses:

http://www.ncbi.nlm.nih.gov/pubmed [nih.gov]
http://www.ncbi.nlm.nih.gov/pubmed/ [nih.gov]</sentencetext>
	<parent>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30908970</parent>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30914994</id>
	<title>bit of an unstory, this</title>
	<author>Anonymous</author>
	<datestamp>1264591200000</datestamp>
	<modclass>None</modclass>
	<modscore>0</modscore>
	<htmltext><p>I finished my PhD in phylogenetics in 2008 and have been a professional bioinformatican since then.</p><p>This story doesn't really suprise me, I'm amazed anyone bothered to write it.</p><p>Yes, we all use bioperl and GPL java libraries, which are free - but that's only because we're all such shit coders (and tbh working on low-complexity, high-specificity tasks - grep, anyone?) that making a proprietary package good enough that everyone else would need to pay for it isn't gonna happen any time soon. But I can't think of any amateurs that I've ever met, or even heard of, that are actually working in this field.</p><p>At the end of the day, like loads of other people have already pointed out, we all rely on sequencing which definitely *isn't* cheap or free. At. All.</p><p>Sorry.</p><p>Joe Parker<br>Oxford University / Kitson Consulting, Ltd.</p></htmltext>
<tokenext>I finished my PhD in phylogenetics in 2008 and have been a professional bioinformatican since then.This story does n't really suprise me , I 'm amazed anyone bothered to write it.Yes , we all use bioperl and GPL java libraries , which are free - but that 's only because we 're all such shit coders ( and tbh working on low-complexity , high-specificity tasks - grep , anyone ?
) that making a proprietary package good enough that everyone else would need to pay for it is n't gon na happen any time soon .
But I ca n't think of any amateurs that I 've ever met , or even heard of , that are actually working in this field.At the end of the day , like loads of other people have already pointed out , we all rely on sequencing which definitely * is n't * cheap or free .
At. All.Sorry.Joe ParkerOxford University / Kitson Consulting , Ltd .</tokentext>
<sentencetext>I finished my PhD in phylogenetics in 2008 and have been a professional bioinformatican since then.This story doesn't really suprise me, I'm amazed anyone bothered to write it.Yes, we all use bioperl and GPL java libraries, which are free - but that's only because we're all such shit coders (and tbh working on low-complexity, high-specificity tasks - grep, anyone?
) that making a proprietary package good enough that everyone else would need to pay for it isn't gonna happen any time soon.
But I can't think of any amateurs that I've ever met, or even heard of, that are actually working in this field.At the end of the day, like loads of other people have already pointed out, we all rely on sequencing which definitely *isn't* cheap or free.
At. All.Sorry.Joe ParkerOxford University / Kitson Consulting, Ltd.</sentencetext>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30913014</id>
	<title>widgets are us</title>
	<author>Anonymous</author>
	<datestamp>1264520520000</datestamp>
	<modclass>None</modclass>
	<modscore>0</modscore>
	<htmltext><p>Well, one element down: elaborate, specialized equipment... if this works, in a few years, you'll just order it from your garage http://www.cubespawn.com</p></htmltext>
<tokenext>Well , one element down : elaborate , specialized equipment... if this works , in a few years , you 'll just order it from your garage http : //www.cubespawn.com</tokentext>
<sentencetext>Well, one element down: elaborate, specialized equipment... if this works, in a few years, you'll just order it from your garage http://www.cubespawn.com</sentencetext>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30912158</id>
	<title>Re:IP is not hobbling traditional research</title>
	<author>geekoid</author>
	<datestamp>1264512900000</datestamp>
	<modclass>None</modclass>
	<modscore>1</modscore>
	<htmltext><p>It is intended that people can build on other patents. The system is specifically set up to do so.</p><p>I can take you patent, 'improve it', and patent the results.</p><p>Here is an example:<br>Lets say you invent a device that thaws meat faster. (basically a piece of steel you put the meat on.</p><p>I can take your device, add a piece that closes onto the top of the meat to thaw both sides at the same time. IT would be a valid patent, and I wouldn't be violating IP.</p></htmltext>
<tokenext>It is intended that people can build on other patents .
The system is specifically set up to do so.I can take you patent , 'improve it ' , and patent the results.Here is an example : Lets say you invent a device that thaws meat faster .
( basically a piece of steel you put the meat on.I can take your device , add a piece that closes onto the top of the meat to thaw both sides at the same time .
IT would be a valid patent , and I would n't be violating IP .</tokentext>
<sentencetext>It is intended that people can build on other patents.
The system is specifically set up to do so.I can take you patent, 'improve it', and patent the results.Here is an example:Lets say you invent a device that thaws meat faster.
(basically a piece of steel you put the meat on.I can take your device, add a piece that closes onto the top of the meat to thaw both sides at the same time.
IT would be a valid patent, and I wouldn't be violating IP.</sentencetext>
	<parent>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30911046</parent>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30912474</id>
	<title>New meaning for "Making a Baby"</title>
	<author>Anonymous</author>
	<datestamp>1264515120000</datestamp>
	<modclass>None</modclass>
	<modscore>0</modscore>
	<htmltext><p>B: Honey, I've got my DIYbio kit and I'm headed to the basement! Don't bother me for a while I'm busy making a baby!<br>A: Don't you need me for that?<br>B: not anymore. not... any... more...</p></htmltext>
<tokenext>B : Honey , I 've got my DIYbio kit and I 'm headed to the basement !
Do n't bother me for a while I 'm busy making a baby ! A : Do n't you need me for that ? B : not anymore .
not... any... more.. .</tokentext>
<sentencetext>B: Honey, I've got my DIYbio kit and I'm headed to the basement!
Don't bother me for a while I'm busy making a baby!A: Don't you need me for that?B: not anymore.
not... any... more...</sentencetext>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30908250</id>
	<title>Intellectual property laws are good</title>
	<author>Anonymous</author>
	<datestamp>1264536360000</datestamp>
	<modclass>None</modclass>
	<modscore>0</modscore>
	<htmltext><p>Intellectual property [law] hobbles research? Actually, intellectual property laws promote research, by giving inventors a means of supporting their work in a competitive marketplace that is independent of government funding. Without IP laws-and without respect for them-the government would wield even more power than now (already too much) and efficiency would suffer even more.</p></htmltext>
<tokenext>Intellectual property [ law ] hobbles research ?
Actually , intellectual property laws promote research , by giving inventors a means of supporting their work in a competitive marketplace that is independent of government funding .
Without IP laws-and without respect for them-the government would wield even more power than now ( already too much ) and efficiency would suffer even more .</tokentext>
<sentencetext>Intellectual property [law] hobbles research?
Actually, intellectual property laws promote research, by giving inventors a means of supporting their work in a competitive marketplace that is independent of government funding.
Without IP laws-and without respect for them-the government would wield even more power than now (already too much) and efficiency would suffer even more.</sentencetext>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30909940</id>
	<title>Re:Kidding, right?</title>
	<author>Zerth</author>
	<datestamp>1264500600000</datestamp>
	<modclass>None</modclass>
	<modscore>1</modscore>
	<htmltext><blockquote><div><p>How do you discard biohazardous materials and mutagen/teratogen substances at home?</p></div></blockquote><p>A handful of rust, a heap of aluminum, mix in a concrete bowl, garnish with a magnesium ribbon, light and cover securely.</p><p>Do wear eye protection, though.</p></div>
	</htmltext>
<tokenext>How do you discard biohazardous materials and mutagen/teratogen substances at home ? A handful of rust , a heap of aluminum , mix in a concrete bowl , garnish with a magnesium ribbon , light and cover securely.Do wear eye protection , though .</tokentext>
<sentencetext>How do you discard biohazardous materials and mutagen/teratogen substances at home?A handful of rust, a heap of aluminum, mix in a concrete bowl, garnish with a magnesium ribbon, light and cover securely.Do wear eye protection, though.
	</sentencetext>
	<parent>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30907610</parent>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30906996</id>
	<title>Re:Uhhh...</title>
	<author>onepoint</author>
	<datestamp>1264531440000</datestamp>
	<modclass>None</modclass>
	<modscore>1</modscore>
	<htmltext><p>this is something that I feel very uncomfortable with, it's the fear of something getting out of the lab, or the worst case example<nobr> <wbr></nobr>.... grey goo</p></htmltext>
<tokenext>this is something that I feel very uncomfortable with , it 's the fear of something getting out of the lab , or the worst case example .... grey goo</tokentext>
<sentencetext>this is something that I feel very uncomfortable with, it's the fear of something getting out of the lab, or the worst case example .... grey goo</sentencetext>
	<parent>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30906858</parent>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30907774</id>
	<title>Been doing that</title>
	<author>edrobinson</author>
	<datestamp>1264534680000</datestamp>
	<modclass>Funny</modclass>
	<modscore>1</modscore>
	<htmltext>The wife and I have been doing DIY biology for years and we have 3 kids and 3 grandkids to show for it.</htmltext>
<tokenext>The wife and I have been doing DIY biology for years and we have 3 kids and 3 grandkids to show for it .</tokentext>
<sentencetext>The wife and I have been doing DIY biology for years and we have 3 kids and 3 grandkids to show for it.</sentencetext>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30909342</id>
	<title>Re:Kidding, right?</title>
	<author>Hatta</author>
	<datestamp>1264498140000</datestamp>
	<modclass>Insightful</modclass>
	<modscore>2</modscore>
	<htmltext><p><i>The only way to do at home what they claim to be doing is by using stuff from their academic research labs.</i></p><p>Not really.  You can get E. coli that express recombinant enzymes and purify it yourself.  And patents don't cover stuff for personal use, so you're clear there.</p><p><i>Besides the risks involved (those cell line are actually cancer cells and engineered bacteria are mutant germs</i></p><p>None of which have a chance to survive outside of carefully controlled laboratory conditions.</p><p><i>not to mention the radioactively labeled nucleic acid probes that might end up in the toilet</i></p><p>I doubt anyone's using radioactive probes at home, probably more fluorescence, chemiluminescence, etc.</p><p><i>Storing liquid nitrogen in your basement? </i></p><p>Not really a problem if you pony up for the right container.</p><p><i>Discarding ethidium-bromide and acrylamide gels? </i></p><p>There are non-toxic stains for agarose gels.  Polymerized acrylamide is not that toxic either.</p><p><i>Biological experiments are different from software development, they need follow up and supervision through the end, which may take 2-4 days. Drosophilla flys can't be frozen like bacteria.</i></p><p>For people dedicated to the hobby, there's no reason they can't deal with that.</p><p><i>How do you discard biohazardous materials and mutagen/teratogen substances at home? </i></p><p>This is a valid concern.  The best way is to find ways to perform experiments that don't require hazardous materials.  There is a lot of biology that does require hazardous materials, but there's also a lot that doesn't.</p></htmltext>
<tokenext>The only way to do at home what they claim to be doing is by using stuff from their academic research labs.Not really .
You can get E. coli that express recombinant enzymes and purify it yourself .
And patents do n't cover stuff for personal use , so you 're clear there.Besides the risks involved ( those cell line are actually cancer cells and engineered bacteria are mutant germsNone of which have a chance to survive outside of carefully controlled laboratory conditions.not to mention the radioactively labeled nucleic acid probes that might end up in the toiletI doubt anyone 's using radioactive probes at home , probably more fluorescence , chemiluminescence , etc.Storing liquid nitrogen in your basement ?
Not really a problem if you pony up for the right container.Discarding ethidium-bromide and acrylamide gels ?
There are non-toxic stains for agarose gels .
Polymerized acrylamide is not that toxic either.Biological experiments are different from software development , they need follow up and supervision through the end , which may take 2-4 days .
Drosophilla flys ca n't be frozen like bacteria.For people dedicated to the hobby , there 's no reason they ca n't deal with that.How do you discard biohazardous materials and mutagen/teratogen substances at home ?
This is a valid concern .
The best way is to find ways to perform experiments that do n't require hazardous materials .
There is a lot of biology that does require hazardous materials , but there 's also a lot that does n't .</tokentext>
<sentencetext>The only way to do at home what they claim to be doing is by using stuff from their academic research labs.Not really.
You can get E. coli that express recombinant enzymes and purify it yourself.
And patents don't cover stuff for personal use, so you're clear there.Besides the risks involved (those cell line are actually cancer cells and engineered bacteria are mutant germsNone of which have a chance to survive outside of carefully controlled laboratory conditions.not to mention the radioactively labeled nucleic acid probes that might end up in the toiletI doubt anyone's using radioactive probes at home, probably more fluorescence, chemiluminescence, etc.Storing liquid nitrogen in your basement?
Not really a problem if you pony up for the right container.Discarding ethidium-bromide and acrylamide gels?
There are non-toxic stains for agarose gels.
Polymerized acrylamide is not that toxic either.Biological experiments are different from software development, they need follow up and supervision through the end, which may take 2-4 days.
Drosophilla flys can't be frozen like bacteria.For people dedicated to the hobby, there's no reason they can't deal with that.How do you discard biohazardous materials and mutagen/teratogen substances at home?
This is a valid concern.
The best way is to find ways to perform experiments that don't require hazardous materials.
There is a lot of biology that does require hazardous materials, but there's also a lot that doesn't.</sentencetext>
	<parent>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30907610</parent>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30922700</id>
	<title>Re:The open source flu</title>
	<author>Anonymous</author>
	<datestamp>1264624980000</datestamp>
	<modclass>None</modclass>
	<modscore>0</modscore>
	<htmltext><p>Yeah, but it's a shallow bug.</p></htmltext>
<tokenext>Yeah , but it 's a shallow bug .</tokentext>
<sentencetext>Yeah, but it's a shallow bug.</sentencetext>
	<parent>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30912124</parent>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30908482</id>
	<title>Good, let's end the War on (some) Drugs..</title>
	<author>Anonymous</author>
	<datestamp>1264537140000</datestamp>
	<modclass>None</modclass>
	<modscore>0</modscore>
	<htmltext><p>.. and create THC-producing dandelions or clover! Seriously.</p></htmltext>
<tokenext>.. and create THC-producing dandelions or clover !
Seriously .</tokentext>
<sentencetext>.. and create THC-producing dandelions or clover!
Seriously.</sentencetext>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30907092</id>
	<title>Any progress?</title>
	<author>vlm</author>
	<datestamp>1264531740000</datestamp>
	<modclass>Insightful</modclass>
	<modscore>4</modscore>
	<htmltext><p>Any progress since the last time this was on slashdot?  No?  Thought so.</p><p>Downloading computational biology software, that you have no idea how to use, makes you a molecular biologist, the same way that downloading finite element analysis software that you don't know how to use, makes you a mechanical engineer, downloading a SPICE simulator that you don't know how to use, makes you an electrical engineer, or downloading Pr0n that you can't re-enact makes you a sex expert.  At least the Pr0n is easier to apply than a FEM or SPICE package, it being a "pictorial diagram", the disadvantage being that it requires a member of the appropriate sex (and species!) to re-enact.</p></htmltext>
<tokenext>Any progress since the last time this was on slashdot ?
No ? Thought so.Downloading computational biology software , that you have no idea how to use , makes you a molecular biologist , the same way that downloading finite element analysis software that you do n't know how to use , makes you a mechanical engineer , downloading a SPICE simulator that you do n't know how to use , makes you an electrical engineer , or downloading Pr0n that you ca n't re-enact makes you a sex expert .
At least the Pr0n is easier to apply than a FEM or SPICE package , it being a " pictorial diagram " , the disadvantage being that it requires a member of the appropriate sex ( and species !
) to re-enact .</tokentext>
<sentencetext>Any progress since the last time this was on slashdot?
No?  Thought so.Downloading computational biology software, that you have no idea how to use, makes you a molecular biologist, the same way that downloading finite element analysis software that you don't know how to use, makes you a mechanical engineer, downloading a SPICE simulator that you don't know how to use, makes you an electrical engineer, or downloading Pr0n that you can't re-enact makes you a sex expert.
At least the Pr0n is easier to apply than a FEM or SPICE package, it being a "pictorial diagram", the disadvantage being that it requires a member of the appropriate sex (and species!
) to re-enact.</sentencetext>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30909234</id>
	<title>Re:Depends</title>
	<author>tburkhol</author>
	<datestamp>1264497660000</datestamp>
	<modclass>None</modclass>
	<modscore>1</modscore>
	<htmltext><p><div class="quote"><p>Many of these biology experiments require very expensive machines, such as microarray machines, as mentioned by the article.</p> </div><p>You'd be surprised what you can accomplish with half-assed equipment.  For example, you don't have to buy Affymetrix latest 30,000 gene chip, you can spot, by hand, a dozen or more probes onto a glass slide and visualize them with a DSLR camera.  The results may not be suitable for Nature (or even your most hated journal), but it's still discovery.  It's discovery you can do in your garage that would have been impossible for a major research lab just 20 years ago.</p><p>That's the point really: a lot of this discovery is based on very straightforward techniques and tools, but they're so sensitive that you can get a pretty good result with care and poor equipment.  Same as the guys who build rockets in their garage: they may not get to orbit; they may not clear the atmosphere, but they can do some exceptionally sophisticated physics without a NASA grant.  If you're doing biology for your own joy, you can do some very sophisticated cell and molecular biology without an NIH grant.</p><p>Tissue?  I passed a dead rat on my way in today - off limits for professional research, but there's nothing preventing an amateur from using it.  Or from taking as much of his own tissue as he'd like.  From his friends, too, if he can convince them it's cool.</p></div>
	</htmltext>
<tokenext>Many of these biology experiments require very expensive machines , such as microarray machines , as mentioned by the article .
You 'd be surprised what you can accomplish with half-assed equipment .
For example , you do n't have to buy Affymetrix latest 30,000 gene chip , you can spot , by hand , a dozen or more probes onto a glass slide and visualize them with a DSLR camera .
The results may not be suitable for Nature ( or even your most hated journal ) , but it 's still discovery .
It 's discovery you can do in your garage that would have been impossible for a major research lab just 20 years ago.That 's the point really : a lot of this discovery is based on very straightforward techniques and tools , but they 're so sensitive that you can get a pretty good result with care and poor equipment .
Same as the guys who build rockets in their garage : they may not get to orbit ; they may not clear the atmosphere , but they can do some exceptionally sophisticated physics without a NASA grant .
If you 're doing biology for your own joy , you can do some very sophisticated cell and molecular biology without an NIH grant.Tissue ?
I passed a dead rat on my way in today - off limits for professional research , but there 's nothing preventing an amateur from using it .
Or from taking as much of his own tissue as he 'd like .
From his friends , too , if he can convince them it 's cool .</tokentext>
<sentencetext>Many of these biology experiments require very expensive machines, such as microarray machines, as mentioned by the article.
You'd be surprised what you can accomplish with half-assed equipment.
For example, you don't have to buy Affymetrix latest 30,000 gene chip, you can spot, by hand, a dozen or more probes onto a glass slide and visualize them with a DSLR camera.
The results may not be suitable for Nature (or even your most hated journal), but it's still discovery.
It's discovery you can do in your garage that would have been impossible for a major research lab just 20 years ago.That's the point really: a lot of this discovery is based on very straightforward techniques and tools, but they're so sensitive that you can get a pretty good result with care and poor equipment.
Same as the guys who build rockets in their garage: they may not get to orbit; they may not clear the atmosphere, but they can do some exceptionally sophisticated physics without a NASA grant.
If you're doing biology for your own joy, you can do some very sophisticated cell and molecular biology without an NIH grant.Tissue?
I passed a dead rat on my way in today - off limits for professional research, but there's nothing preventing an amateur from using it.
Or from taking as much of his own tissue as he'd like.
From his friends, too, if he can convince them it's cool.
	</sentencetext>
	<parent>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30907022</parent>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30912178</id>
	<title>Re:Uhhh...</title>
	<author>geekoid</author>
	<datestamp>1264513080000</datestamp>
	<modclass>None</modclass>
	<modscore>1</modscore>
	<htmltext><p>If you can't understand IKEA instructions, then DIY biology IS then only kind your allowed to have.</p></htmltext>
<tokenext>If you ca n't understand IKEA instructions , then DIY biology IS then only kind your allowed to have .</tokentext>
<sentencetext>If you can't understand IKEA instructions, then DIY biology IS then only kind your allowed to have.</sentencetext>
	<parent>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30906858</parent>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30910882</id>
	<title>Re:Kidding, right?</title>
	<author>bwv549</author>
	<datestamp>1264504680000</datestamp>
	<modclass>None</modclass>
	<modscore>1</modscore>
	<htmltext>DIYers discovering and/or promoting low risk/cost solutions to problems in experimental biology sounds like a big win for everyone (including those in academic/industrial settings).</htmltext>
<tokenext>DIYers discovering and/or promoting low risk/cost solutions to problems in experimental biology sounds like a big win for everyone ( including those in academic/industrial settings ) .</tokentext>
<sentencetext>DIYers discovering and/or promoting low risk/cost solutions to problems in experimental biology sounds like a big win for everyone (including those in academic/industrial settings).</sentencetext>
	<parent>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30907610</parent>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30909584</id>
	<title>Re:Kidding, right?</title>
	<author>Anonymous</author>
	<datestamp>1264499100000</datestamp>
	<modclass>None</modclass>
	<modscore>0</modscore>
	<htmltext><p><div class="quote"><p>Forget the open-source/custom-made software and discount price hardware acquired on eBay, biology is first and foremost about wet lab. And not only it costs *a lot* but one needs licenses to purchase certain products.</p></div><p>I don't think anyone's arguing that someone working out of his garage can do first class, publishable research.  If you're just looking for results, you don't have to use triple-purified, ultra-certified AmpliTaq Platinum.  You can extract and purify enzymes of modest activity from your own rainwater-derived bacterial colonies or grocery store chicken.  Jesus, man, do you even know where those "certain products" that require licenses came from in the first place?</p><p><div class="quote"><p>Discarding ethidium-bromide and acrylamide gels?</p></div><p>Do you know ethidium bromide is used to treat livestock parasites?  Do you know contact lenses are made of polyacyrlamide?  How do you dispose of the sodium hypochlorite in your home laundry?</p></div>
	</htmltext>
<tokenext>Forget the open-source/custom-made software and discount price hardware acquired on eBay , biology is first and foremost about wet lab .
And not only it costs * a lot * but one needs licenses to purchase certain products.I do n't think anyone 's arguing that someone working out of his garage can do first class , publishable research .
If you 're just looking for results , you do n't have to use triple-purified , ultra-certified AmpliTaq Platinum .
You can extract and purify enzymes of modest activity from your own rainwater-derived bacterial colonies or grocery store chicken .
Jesus , man , do you even know where those " certain products " that require licenses came from in the first place ? Discarding ethidium-bromide and acrylamide gels ? Do you know ethidium bromide is used to treat livestock parasites ?
Do you know contact lenses are made of polyacyrlamide ?
How do you dispose of the sodium hypochlorite in your home laundry ?</tokentext>
<sentencetext>Forget the open-source/custom-made software and discount price hardware acquired on eBay, biology is first and foremost about wet lab.
And not only it costs *a lot* but one needs licenses to purchase certain products.I don't think anyone's arguing that someone working out of his garage can do first class, publishable research.
If you're just looking for results, you don't have to use triple-purified, ultra-certified AmpliTaq Platinum.
You can extract and purify enzymes of modest activity from your own rainwater-derived bacterial colonies or grocery store chicken.
Jesus, man, do you even know where those "certain products" that require licenses came from in the first place?Discarding ethidium-bromide and acrylamide gels?Do you know ethidium bromide is used to treat livestock parasites?
Do you know contact lenses are made of polyacyrlamide?
How do you dispose of the sodium hypochlorite in your home laundry?
	</sentencetext>
	<parent>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30907610</parent>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30907418</id>
	<title>what's needed</title>
	<author>Anonymous</author>
	<datestamp>1264533240000</datestamp>
	<modclass>None</modclass>
	<modscore>0</modscore>
	<htmltext><p>I tell you what's sorely needed: an open-source biology that should be to Monsanto what Linux is to Microsoft.</p><p>AC</p></htmltext>
<tokenext>I tell you what 's sorely needed : an open-source biology that should be to Monsanto what Linux is to Microsoft.AC</tokentext>
<sentencetext>I tell you what's sorely needed: an open-source biology that should be to Monsanto what Linux is to Microsoft.AC</sentencetext>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30907540</id>
	<title>Re:Uhhh...</title>
	<author>Hurricane78</author>
	<datestamp>1264533720000</datestamp>
	<modclass>Funny</modclass>
	<modscore>3</modscore>
	<htmltext><p>Excuse me. In our days, we used to call that any of:<br>- Sex<br>- Pregnancy<br>- Having mold in the bathroom/basement/etc.<br>- Growing your own food.<br>- Brewing your own beer / making your own cheese/salami/etc.<br>- Letting the dog lick your face.<br>- Actually eating the sand-cake that you made in the sandbox where the dogs used to poop.<br>And we lived with it! (Not in that order, though.<nobr> <wbr></nobr>;)</p><p>The youth today. A bunch of bubble boys in fear of the world.<br>Now get off my lawn!</p></htmltext>
<tokenext>Excuse me .
In our days , we used to call that any of : - Sex- Pregnancy- Having mold in the bathroom/basement/etc.- Growing your own food.- Brewing your own beer / making your own cheese/salami/etc.- Letting the dog lick your face.- Actually eating the sand-cake that you made in the sandbox where the dogs used to poop.And we lived with it !
( Not in that order , though .
; ) The youth today .
A bunch of bubble boys in fear of the world.Now get off my lawn !</tokentext>
<sentencetext>Excuse me.
In our days, we used to call that any of:- Sex- Pregnancy- Having mold in the bathroom/basement/etc.- Growing your own food.- Brewing your own beer / making your own cheese/salami/etc.- Letting the dog lick your face.- Actually eating the sand-cake that you made in the sandbox where the dogs used to poop.And we lived with it!
(Not in that order, though.
;)The youth today.
A bunch of bubble boys in fear of the world.Now get off my lawn!</sentencetext>
	<parent>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30906858</parent>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30907362</id>
	<title>Folding@Home is not a "DIY" project. . .</title>
	<author>the gnat</author>
	<datestamp>1264532940000</datestamp>
	<modclass>Insightful</modclass>
	<modscore>5</modscore>
	<htmltext><p>Vijay Pande is a Stanford professor and funded primarily by the same agencies that fund most of the biomedical research in this country - most importantly, the NIH.  (Disclaimer: they fund my work too.)  He has full-time scientists (i.e. people who spent most of their 20s in school) and computer engineers writing code and assistance from hardware vendors (ATI/AMD and NVIDIA, at least).  FAH is a great example of how to leverage distributed computing resources and volunteer effort, and it's an excellent technical solution to what is potentially a very expensive problem, but the intellectual effort is *not* distributed.  I don't mean any of this as a criticism (I wish I had five petaflops at my disposal too), but this is not an example of "hobbyists" performing research free of bureaucracy.  (In fact, the umbrella project for much of Pande's work now has a relatively large bureaucracy at Stanford, which surely wasn't suffering from a lack of bureaucracy to begin with.)</p></htmltext>
<tokenext>Vijay Pande is a Stanford professor and funded primarily by the same agencies that fund most of the biomedical research in this country - most importantly , the NIH .
( Disclaimer : they fund my work too .
) He has full-time scientists ( i.e .
people who spent most of their 20s in school ) and computer engineers writing code and assistance from hardware vendors ( ATI/AMD and NVIDIA , at least ) .
FAH is a great example of how to leverage distributed computing resources and volunteer effort , and it 's an excellent technical solution to what is potentially a very expensive problem , but the intellectual effort is * not * distributed .
I do n't mean any of this as a criticism ( I wish I had five petaflops at my disposal too ) , but this is not an example of " hobbyists " performing research free of bureaucracy .
( In fact , the umbrella project for much of Pande 's work now has a relatively large bureaucracy at Stanford , which surely was n't suffering from a lack of bureaucracy to begin with .
)</tokentext>
<sentencetext>Vijay Pande is a Stanford professor and funded primarily by the same agencies that fund most of the biomedical research in this country - most importantly, the NIH.
(Disclaimer: they fund my work too.
)  He has full-time scientists (i.e.
people who spent most of their 20s in school) and computer engineers writing code and assistance from hardware vendors (ATI/AMD and NVIDIA, at least).
FAH is a great example of how to leverage distributed computing resources and volunteer effort, and it's an excellent technical solution to what is potentially a very expensive problem, but the intellectual effort is *not* distributed.
I don't mean any of this as a criticism (I wish I had five petaflops at my disposal too), but this is not an example of "hobbyists" performing research free of bureaucracy.
(In fact, the umbrella project for much of Pande's work now has a relatively large bureaucracy at Stanford, which surely wasn't suffering from a lack of bureaucracy to begin with.
)</sentencetext>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30907114</id>
	<title>It's bad enough here with electronics hardware.</title>
	<author>Anonymous</author>
	<datestamp>1264531860000</datestamp>
	<modclass>Interestin</modclass>
	<modscore>2</modscore>
	<htmltext><p>A poll recently indicated 95\%+ coders here.  Something about computer science makes comments skew strangely.  Look at an article on encryption, and you'll get quite a few accurate, thoughtful comments.  Look at one on CPUs, or applied physics, and you got a lot of jokes and misunderstandings.  Is there something peculiar about the field of computer science that makes a worldview tilted so much?</p></htmltext>
<tokenext>A poll recently indicated 95 \ % + coders here .
Something about computer science makes comments skew strangely .
Look at an article on encryption , and you 'll get quite a few accurate , thoughtful comments .
Look at one on CPUs , or applied physics , and you got a lot of jokes and misunderstandings .
Is there something peculiar about the field of computer science that makes a worldview tilted so much ?</tokentext>
<sentencetext>A poll recently indicated 95\%+ coders here.
Something about computer science makes comments skew strangely.
Look at an article on encryption, and you'll get quite a few accurate, thoughtful comments.
Look at one on CPUs, or applied physics, and you got a lot of jokes and misunderstandings.
Is there something peculiar about the field of computer science that makes a worldview tilted so much?</sentencetext>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30914804</id>
	<title>Re:Uhhh...</title>
	<author>Anonymous</author>
	<datestamp>1264587840000</datestamp>
	<modclass>None</modclass>
	<modscore>0</modscore>
	<htmltext><p><div class="quote"><p>DIY Biology sounds pretty dangerous.</p><p>As long as the instructions it comes with are better than Ikea's...</p></div><p>I would suggest that if Ikea's instructions give you trouble then maybe Biology aint your thing<nobr> <wbr></nobr>:-)</p></div>
	</htmltext>
<tokenext>DIY Biology sounds pretty dangerous.As long as the instructions it comes with are better than Ikea 's...I would suggest that if Ikea 's instructions give you trouble then maybe Biology aint your thing : - )</tokentext>
<sentencetext>DIY Biology sounds pretty dangerous.As long as the instructions it comes with are better than Ikea's...I would suggest that if Ikea's instructions give you trouble then maybe Biology aint your thing :-)
	</sentencetext>
	<parent>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30906858</parent>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30908452</id>
	<title>Re:Uhhh...</title>
	<author>interkin3tic</author>
	<datestamp>1264537020000</datestamp>
	<modclass>Insightful</modclass>
	<modscore>2</modscore>
	<htmltext><p>Probably a bit pedantic, but <a href="http://en.wikipedia.org/wiki/Grey\_goo" title="wikipedia.org">grey goo</a> [wikipedia.org] means nanobots out of control.  You're thinking of biological threats, like artificial superflu or <a href="http://en.wikipedia.org/wiki/Reston\_ebolavirus" title="wikipedia.org">ebola reston</a> [wikipedia.org] mutated to become pathogenic to humans or something similar, which I guess would be green goo?</p><p>Grey goo technically wouldn't be a product of DIY biology, that would be DIY nanotech.</p><p>Probably a bigger concern is invasive GMO taking over, but this I think is a bigger concern from say Monsanto, which has more money to put into making GMOs and seems a lot less concerned with ethics or long term consequences than individual researchers.  If they were to find some genes that allowed plants to outcompete any wild plant, and it got out into the wild, it might be difficult/expensive to contain.  Outside of several plant labs you can find <a href="http://en.wikipedia.org/wiki/Arabidopsis" title="wikipedia.org">Arabidopsis</a> [wikipedia.org] that "got out", some could be harmless GMOs.  I could easily see monsanto making a superplant arabidopsis and then being careless with the seeds.</p><p>I should state that I'm not a plant biologist, don't work at monsanto, and have no idea what if any legal or technical restrictions are in place to prevent that, they could be good ones.  I'm just saying I'm more worried about dangerous biological threats coming out of corporate labs than someones garage.</p></htmltext>
<tokenext>Probably a bit pedantic , but grey goo [ wikipedia.org ] means nanobots out of control .
You 're thinking of biological threats , like artificial superflu or ebola reston [ wikipedia.org ] mutated to become pathogenic to humans or something similar , which I guess would be green goo ? Grey goo technically would n't be a product of DIY biology , that would be DIY nanotech.Probably a bigger concern is invasive GMO taking over , but this I think is a bigger concern from say Monsanto , which has more money to put into making GMOs and seems a lot less concerned with ethics or long term consequences than individual researchers .
If they were to find some genes that allowed plants to outcompete any wild plant , and it got out into the wild , it might be difficult/expensive to contain .
Outside of several plant labs you can find Arabidopsis [ wikipedia.org ] that " got out " , some could be harmless GMOs .
I could easily see monsanto making a superplant arabidopsis and then being careless with the seeds.I should state that I 'm not a plant biologist , do n't work at monsanto , and have no idea what if any legal or technical restrictions are in place to prevent that , they could be good ones .
I 'm just saying I 'm more worried about dangerous biological threats coming out of corporate labs than someones garage .</tokentext>
<sentencetext>Probably a bit pedantic, but grey goo [wikipedia.org] means nanobots out of control.
You're thinking of biological threats, like artificial superflu or ebola reston [wikipedia.org] mutated to become pathogenic to humans or something similar, which I guess would be green goo?Grey goo technically wouldn't be a product of DIY biology, that would be DIY nanotech.Probably a bigger concern is invasive GMO taking over, but this I think is a bigger concern from say Monsanto, which has more money to put into making GMOs and seems a lot less concerned with ethics or long term consequences than individual researchers.
If they were to find some genes that allowed plants to outcompete any wild plant, and it got out into the wild, it might be difficult/expensive to contain.
Outside of several plant labs you can find Arabidopsis [wikipedia.org] that "got out", some could be harmless GMOs.
I could easily see monsanto making a superplant arabidopsis and then being careless with the seeds.I should state that I'm not a plant biologist, don't work at monsanto, and have no idea what if any legal or technical restrictions are in place to prevent that, they could be good ones.
I'm just saying I'm more worried about dangerous biological threats coming out of corporate labs than someones garage.</sentencetext>
	<parent>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30906996</parent>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30909096</id>
	<title>Re:Kidding, right?</title>
	<author>St.Creed</author>
	<datestamp>1264497000000</datestamp>
	<modclass>None</modclass>
	<modscore>1</modscore>
	<htmltext><p><div class="quote"><p> How do you discard biohazardous materials and mutagen/teratogen substances at home?</p> </div><p>Down the toilet, flush twice.<nobr> <wbr></nobr>:)</p></div>
	</htmltext>
<tokenext>How do you discard biohazardous materials and mutagen/teratogen substances at home ?
Down the toilet , flush twice .
: )</tokentext>
<sentencetext> How do you discard biohazardous materials and mutagen/teratogen substances at home?
Down the toilet, flush twice.
:)
	</sentencetext>
	<parent>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30907610</parent>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30909354</id>
	<title>Re:IP is not hobbling traditional research</title>
	<author>Anonymous</author>
	<datestamp>1264498200000</datestamp>
	<modclass>None</modclass>
	<modscore>0</modscore>
	<htmltext><p>I doubt that the harm happens at the level of actualised research, but rather research choices are effected by intellectual property. Thus, it slips by this study relatively unnoticed.</p></htmltext>
<tokenext>I doubt that the harm happens at the level of actualised research , but rather research choices are effected by intellectual property .
Thus , it slips by this study relatively unnoticed .</tokentext>
<sentencetext>I doubt that the harm happens at the level of actualised research, but rather research choices are effected by intellectual property.
Thus, it slips by this study relatively unnoticed.</sentencetext>
	<parent>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30907406</parent>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30910710</id>
	<title>Re:Uhhh...</title>
	<author>LifesABeach</author>
	<datestamp>1264503780000</datestamp>
	<modclass>None</modclass>
	<modscore>1</modscore>
	<htmltext>Funny, I was thinking of the DIY Biologists working on a mutagen that would reverse the "age" of skin cells for the purpose of avoiding such accidents, and <a href="http://www.nordstroms.com/" title="nordstroms.com">Nordstoms Semi Annual Sales.</a> [nordstroms.com]</htmltext>
<tokenext>Funny , I was thinking of the DIY Biologists working on a mutagen that would reverse the " age " of skin cells for the purpose of avoiding such accidents , and Nordstoms Semi Annual Sales .
[ nordstroms.com ]</tokentext>
<sentencetext>Funny, I was thinking of the DIY Biologists working on a mutagen that would reverse the "age" of skin cells for the purpose of avoiding such accidents, and Nordstoms Semi Annual Sales.
[nordstroms.com]</sentencetext>
	<parent>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30907050</parent>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30907432</id>
	<title>Re:Uhhh...</title>
	<author>Chris Mattern</author>
	<datestamp>1264533240000</datestamp>
	<modclass>Insightful</modclass>
	<modscore>2</modscore>
	<htmltext><p>I actually flagged this article "graygoo" myself, but in fact, it's not as likely as a lot of people think.  The microbial ecology of the earth is a battlefield, with each micro-organism looking to expand its niche at the expense of others.  Our would-be gray goo organism isn't going to take over the earth--it's going to get mugged for its lunch money and its carcass eaten by whatever can find nutrient value in it.</p></htmltext>
<tokenext>I actually flagged this article " graygoo " myself , but in fact , it 's not as likely as a lot of people think .
The microbial ecology of the earth is a battlefield , with each micro-organism looking to expand its niche at the expense of others .
Our would-be gray goo organism is n't going to take over the earth--it 's going to get mugged for its lunch money and its carcass eaten by whatever can find nutrient value in it .</tokentext>
<sentencetext>I actually flagged this article "graygoo" myself, but in fact, it's not as likely as a lot of people think.
The microbial ecology of the earth is a battlefield, with each micro-organism looking to expand its niche at the expense of others.
Our would-be gray goo organism isn't going to take over the earth--it's going to get mugged for its lunch money and its carcass eaten by whatever can find nutrient value in it.</sentencetext>
	<parent>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30906996</parent>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30907310</id>
	<title>Re:Any progress?</title>
	<author>dangitman</author>
	<datestamp>1264532640000</datestamp>
	<modclass>None</modclass>
	<modscore>1</modscore>
	<htmltext><p><div class="quote"><p>requires a member of the appropriate sex (and species!)</p></div><p>Damn slashdot nerds, always nit-picking.</p></div>
	</htmltext>
<tokenext>requires a member of the appropriate sex ( and species !
) Damn slashdot nerds , always nit-picking .</tokentext>
<sentencetext>requires a member of the appropriate sex (and species!
)Damn slashdot nerds, always nit-picking.
	</sentencetext>
	<parent>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30907092</parent>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30908970</id>
	<title>R Bioconductor Cytoscape EGAN</title>
	<author>bzdyelnik</author>
	<datestamp>1264496400000</datestamp>
	<modclass>Informativ</modclass>
	<modscore>3</modscore>
	<htmltext>Don't forget R/Bioconductor!  Not only is R free/free, but there are thousands of available Bioconductor packages ready for out-of-the-box use.

Also consider Cytoscape and or EGAN for graph visualization of established and experimental bio-knowledge.

<a href="http://www.bioconductor.org/" title="bioconductor.org" rel="nofollow">http://www.bioconductor.org/</a> [bioconductor.org]
<a href="http://www.cytoscape.org/" title="cytoscape.org" rel="nofollow">http://www.cytoscape.org/</a> [cytoscape.org]
<a href="http://akt.ucsf.edu/EGAN/" title="ucsf.edu" rel="nofollow">http://akt.ucsf.edu/EGAN/</a> [ucsf.edu]

(full disclosure - I work on EGAN)</htmltext>
<tokenext>Do n't forget R/Bioconductor !
Not only is R free/free , but there are thousands of available Bioconductor packages ready for out-of-the-box use .
Also consider Cytoscape and or EGAN for graph visualization of established and experimental bio-knowledge .
http : //www.bioconductor.org/ [ bioconductor.org ] http : //www.cytoscape.org/ [ cytoscape.org ] http : //akt.ucsf.edu/EGAN/ [ ucsf.edu ] ( full disclosure - I work on EGAN )</tokentext>
<sentencetext>Don't forget R/Bioconductor!
Not only is R free/free, but there are thousands of available Bioconductor packages ready for out-of-the-box use.
Also consider Cytoscape and or EGAN for graph visualization of established and experimental bio-knowledge.
http://www.bioconductor.org/ [bioconductor.org]
http://www.cytoscape.org/ [cytoscape.org]
http://akt.ucsf.edu/EGAN/ [ucsf.edu]

(full disclosure - I work on EGAN)</sentencetext>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30913260</id>
	<title>Re:Kidding, right?</title>
	<author>Corson</author>
	<datestamp>1264523280000</datestamp>
	<modclass>None</modclass>
	<modscore>1</modscore>
	<htmltext>Guys, if ethidium bromide and polymerized acrylamide were not hazardous then you wouldn't manipulate them wearing rubber gloves, a lab coat, and a protective mask. If bacteria didn't mutate spontaneously then selection wouldn't happen; remember how strains commonly used in the research lab were obtained in the first place. Don't forget that most B cells, primary or cell lines, carry the Ebstein-Barr virus, and that bacteria used for cloning were transformed with vectors carrying antibiotic-resistance genes. IMO at least some of the people posting here should not be allowed to get their hands on biological materials. I still keep a few dozen DNA vectors in TBS in my home fridge, for old times' sake, but they can't do any damage there. This discussion reminds my of a joke: A guy asks a surgeon how difficult it is to learn to do an appendicectomy. The surgeon replies: "Oh, that would be easy. It would take me about fifteen minutes to teach you how to do an appendicectomy. But it would take me four years to teach you what to do should anything go wrong."</htmltext>
<tokenext>Guys , if ethidium bromide and polymerized acrylamide were not hazardous then you would n't manipulate them wearing rubber gloves , a lab coat , and a protective mask .
If bacteria did n't mutate spontaneously then selection would n't happen ; remember how strains commonly used in the research lab were obtained in the first place .
Do n't forget that most B cells , primary or cell lines , carry the Ebstein-Barr virus , and that bacteria used for cloning were transformed with vectors carrying antibiotic-resistance genes .
IMO at least some of the people posting here should not be allowed to get their hands on biological materials .
I still keep a few dozen DNA vectors in TBS in my home fridge , for old times ' sake , but they ca n't do any damage there .
This discussion reminds my of a joke : A guy asks a surgeon how difficult it is to learn to do an appendicectomy .
The surgeon replies : " Oh , that would be easy .
It would take me about fifteen minutes to teach you how to do an appendicectomy .
But it would take me four years to teach you what to do should anything go wrong .
"</tokentext>
<sentencetext>Guys, if ethidium bromide and polymerized acrylamide were not hazardous then you wouldn't manipulate them wearing rubber gloves, a lab coat, and a protective mask.
If bacteria didn't mutate spontaneously then selection wouldn't happen; remember how strains commonly used in the research lab were obtained in the first place.
Don't forget that most B cells, primary or cell lines, carry the Ebstein-Barr virus, and that bacteria used for cloning were transformed with vectors carrying antibiotic-resistance genes.
IMO at least some of the people posting here should not be allowed to get their hands on biological materials.
I still keep a few dozen DNA vectors in TBS in my home fridge, for old times' sake, but they can't do any damage there.
This discussion reminds my of a joke: A guy asks a surgeon how difficult it is to learn to do an appendicectomy.
The surgeon replies: "Oh, that would be easy.
It would take me about fifteen minutes to teach you how to do an appendicectomy.
But it would take me four years to teach you what to do should anything go wrong.
"</sentencetext>
	<parent>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30907610</parent>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30910544</id>
	<title>Re:DIY??</title>
	<author>baKanale</author>
	<datestamp>1264503060000</datestamp>
	<modclass>None</modclass>
	<modscore>1</modscore>
	<htmltext>But do-it-yourself is the Slashdot way!</htmltext>
<tokenext>But do-it-yourself is the Slashdot way !</tokentext>
<sentencetext>But do-it-yourself is the Slashdot way!</sentencetext>
	<parent>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30907066</parent>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30906858</id>
	<title>Uhhh...</title>
	<author>Anonymous</author>
	<datestamp>1264530780000</datestamp>
	<modclass>Funny</modclass>
	<modscore>4</modscore>
	<htmltext><p>DIY Biology sounds pretty dangerous.</p><p>As long as the instructions it comes with are better than Ikea's...</p></htmltext>
<tokenext>DIY Biology sounds pretty dangerous.As long as the instructions it comes with are better than Ikea 's.. .</tokentext>
<sentencetext>DIY Biology sounds pretty dangerous.As long as the instructions it comes with are better than Ikea's...</sentencetext>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30907610</id>
	<title>Kidding, right?</title>
	<author>Anonymous</author>
	<datestamp>1264534020000</datestamp>
	<modclass>Insightful</modclass>
	<modscore>3</modscore>
	<htmltext><strong>"many DIYers knowledge of these fields is so complete that the best among them design and conduct their own experiments at stunningly low costs. With adequate knowledge and ingenuity, DIYbiologists can build equipment and run experiments on a hobbyist's budget.</strong>"

That must be a (bad) joke. Forget the open-source/custom-made software and discount price hardware acquired on eBay, biology is first and foremost about wet lab. And not only it costs *a lot* but one needs licenses to purchase certain products. I have worked in biomedical research for almost ten years and I know that if you're in academia then you can purchase, say, enzymes and genetic vectors at their catalog price; but if you're industry then you get hit with 5-6 digit licensing fees. The only way to do at home what they claim to be doing is by using stuff from their academic research labs. Besides the risks involved (those cell line are actually cancer cells and engineered bacteria are mutant germs, not to mention the radioactively labeled nucleic acid probes that might end up in the toilet) the logistics are a nightmare. Storing liquid nitrogen in your basement? Discarding ethidium-bromide and acrylamide gels? Biological experiments are different from software development, they need follow up and supervision through the end, which may take 2-4 days. Drosophilla flys can't be frozen like bacteria. How do you discard biohazardous materials and mutagen/teratogen substances at home? There are many reasons why DIY biology is a very bad idea; it's a disaster waiting to happen.</htmltext>
<tokenext>" many DIYers knowledge of these fields is so complete that the best among them design and conduct their own experiments at stunningly low costs .
With adequate knowledge and ingenuity , DIYbiologists can build equipment and run experiments on a hobbyist 's budget .
" That must be a ( bad ) joke .
Forget the open-source/custom-made software and discount price hardware acquired on eBay , biology is first and foremost about wet lab .
And not only it costs * a lot * but one needs licenses to purchase certain products .
I have worked in biomedical research for almost ten years and I know that if you 're in academia then you can purchase , say , enzymes and genetic vectors at their catalog price ; but if you 're industry then you get hit with 5-6 digit licensing fees .
The only way to do at home what they claim to be doing is by using stuff from their academic research labs .
Besides the risks involved ( those cell line are actually cancer cells and engineered bacteria are mutant germs , not to mention the radioactively labeled nucleic acid probes that might end up in the toilet ) the logistics are a nightmare .
Storing liquid nitrogen in your basement ?
Discarding ethidium-bromide and acrylamide gels ?
Biological experiments are different from software development , they need follow up and supervision through the end , which may take 2-4 days .
Drosophilla flys ca n't be frozen like bacteria .
How do you discard biohazardous materials and mutagen/teratogen substances at home ?
There are many reasons why DIY biology is a very bad idea ; it 's a disaster waiting to happen .</tokentext>
<sentencetext>"many DIYers knowledge of these fields is so complete that the best among them design and conduct their own experiments at stunningly low costs.
With adequate knowledge and ingenuity, DIYbiologists can build equipment and run experiments on a hobbyist's budget.
"

That must be a (bad) joke.
Forget the open-source/custom-made software and discount price hardware acquired on eBay, biology is first and foremost about wet lab.
And not only it costs *a lot* but one needs licenses to purchase certain products.
I have worked in biomedical research for almost ten years and I know that if you're in academia then you can purchase, say, enzymes and genetic vectors at their catalog price; but if you're industry then you get hit with 5-6 digit licensing fees.
The only way to do at home what they claim to be doing is by using stuff from their academic research labs.
Besides the risks involved (those cell line are actually cancer cells and engineered bacteria are mutant germs, not to mention the radioactively labeled nucleic acid probes that might end up in the toilet) the logistics are a nightmare.
Storing liquid nitrogen in your basement?
Discarding ethidium-bromide and acrylamide gels?
Biological experiments are different from software development, they need follow up and supervision through the end, which may take 2-4 days.
Drosophilla flys can't be frozen like bacteria.
How do you discard biohazardous materials and mutagen/teratogen substances at home?
There are many reasons why DIY biology is a very bad idea; it's a disaster waiting to happen.</sentencetext>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30909618</id>
	<title>Re:Kidding, right?</title>
	<author>marcosdumay</author>
	<datestamp>1264499280000</datestamp>
	<modclass>None</modclass>
	<modscore>1</modscore>
	<htmltext><blockquote><div><p>"Storing liquid nitrogen in your basement? "</p></div> </blockquote><p>Farmers have being storing liquid nitrogen on their (rural area) basement for a few decades now, with very few problems.</p></div>
	</htmltext>
<tokenext>" Storing liquid nitrogen in your basement ?
" Farmers have being storing liquid nitrogen on their ( rural area ) basement for a few decades now , with very few problems .</tokentext>
<sentencetext>"Storing liquid nitrogen in your basement?
" Farmers have being storing liquid nitrogen on their (rural area) basement for a few decades now, with very few problems.
	</sentencetext>
	<parent>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30907610</parent>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30907234</id>
	<title>Stop it!</title>
	<author>Anonymous</author>
	<datestamp>1264532280000</datestamp>
	<modclass>Interestin</modclass>
	<modscore>0</modscore>
	<htmltext><p>"As the shift to open source software continues..."</p><p>Stop the shit, I'm going blind from it, stop it!</p><p>Oh... shiFt... Nevermind, continue, continue, good job...</p></htmltext>
<tokenext>" As the shift to open source software continues... " Stop the shit , I 'm going blind from it , stop it ! Oh... shiFt... Nevermind , continue , continue , good job.. .</tokentext>
<sentencetext>"As the shift to open source software continues..."Stop the shit, I'm going blind from it, stop it!Oh... shiFt... Nevermind, continue, continue, good job...</sentencetext>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30907148</id>
	<title>SpongE</title>
	<author>Anonymous</author>
	<datestamp>1264531980000</datestamp>
	<modclass>Offtopic</modclass>
	<modscore>-1</modscore>
	<htmltext>I'm discus5ing on my Pentium Pro market. Therefore session and join in least I won't</htmltext>
<tokenext>I 'm discus5ing on my Pentium Pro market .
Therefore session and join in least I wo n't</tokentext>
<sentencetext>I'm discus5ing on my Pentium Pro market.
Therefore session and join in least I won't</sentencetext>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30907406</id>
	<title>IP is not hobbling traditional research</title>
	<author>Grond</author>
	<datestamp>1264533180000</datestamp>
	<modclass>Informativ</modclass>
	<modscore>4</modscore>
	<htmltext><p>The article makes some vague statements that IP limits traditional biotech research.  In fact, empirical studies do not back up such claims. John Walsh, Charlene Cho, Wesley Cohen, <em> <a href="http://www.rhsmith.umd.edu/seminars/pdfs/cohen2.pdf" title="umd.edu">View from the Bench: Patents and Material Transfers</a> [umd.edu] </em>, 309 Science 2002-2003 (2005).  Some highlights:</p><p>"Thus, of 381 academic scientists, even including the 10\% who claimed to be doing drug development or related downstream work, none were stopped by the existence of third-party patents, and even modifications or delays were rare, each affecting around 1\% of our sample."</p><p>"In addition, 22 of the 23 respondents to our question about costs reported that there was no fee for the patented technology, and the 23rd respondent said the fee was in the range of $1 to $100."</p><p>19\% of the respondents reported that other scientists had not complied with material transfer requests (i.e. requests for data or samples), but analysis found that "The patent status of the requested material had no significant effect on noncompliance."</p><p>An additional, more focused case study of a highly-commercialized area of research with a lot of patent activity found that "only 3\% of respondents reported stopping a project in the past 2 years because of a patent."</p></htmltext>
<tokenext>The article makes some vague statements that IP limits traditional biotech research .
In fact , empirical studies do not back up such claims .
John Walsh , Charlene Cho , Wesley Cohen , View from the Bench : Patents and Material Transfers [ umd.edu ] , 309 Science 2002-2003 ( 2005 ) .
Some highlights : " Thus , of 381 academic scientists , even including the 10 \ % who claimed to be doing drug development or related downstream work , none were stopped by the existence of third-party patents , and even modifications or delays were rare , each affecting around 1 \ % of our sample .
" " In addition , 22 of the 23 respondents to our question about costs reported that there was no fee for the patented technology , and the 23rd respondent said the fee was in the range of $ 1 to $ 100 .
" 19 \ % of the respondents reported that other scientists had not complied with material transfer requests ( i.e .
requests for data or samples ) , but analysis found that " The patent status of the requested material had no significant effect on noncompliance .
" An additional , more focused case study of a highly-commercialized area of research with a lot of patent activity found that " only 3 \ % of respondents reported stopping a project in the past 2 years because of a patent .
"</tokentext>
<sentencetext>The article makes some vague statements that IP limits traditional biotech research.
In fact, empirical studies do not back up such claims.
John Walsh, Charlene Cho, Wesley Cohen,  View from the Bench: Patents and Material Transfers [umd.edu] , 309 Science 2002-2003 (2005).
Some highlights:"Thus, of 381 academic scientists, even including the 10\% who claimed to be doing drug development or related downstream work, none were stopped by the existence of third-party patents, and even modifications or delays were rare, each affecting around 1\% of our sample.
""In addition, 22 of the 23 respondents to our question about costs reported that there was no fee for the patented technology, and the 23rd respondent said the fee was in the range of $1 to $100.
"19\% of the respondents reported that other scientists had not complied with material transfer requests (i.e.
requests for data or samples), but analysis found that "The patent status of the requested material had no significant effect on noncompliance.
"An additional, more focused case study of a highly-commercialized area of research with a lot of patent activity found that "only 3\% of respondents reported stopping a project in the past 2 years because of a patent.
"</sentencetext>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30907198</id>
	<title>i like cooperative do-it-yourself biology</title>
	<author>Anonymous</author>
	<datestamp>1264532160000</datestamp>
	<modclass>Funny</modclass>
	<modscore>2</modscore>
	<htmltext><p>sadly, i mostly encounter uncooperatives</p><p>this leads to do-it-by-yourself biology</p></htmltext>
<tokenext>sadly , i mostly encounter uncooperativesthis leads to do-it-by-yourself biology</tokentext>
<sentencetext>sadly, i mostly encounter uncooperativesthis leads to do-it-by-yourself biology</sentencetext>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30909474</id>
	<title>Re:DIY??</title>
	<author>Anonymous</author>
	<datestamp>1264498740000</datestamp>
	<modclass>None</modclass>
	<modscore>0</modscore>
	<htmltext>This is<nobr> <wbr></nobr>/. we all are DIY'ers here.</htmltext>
<tokenext>This is / .
we all are DIY'ers here .</tokentext>
<sentencetext>This is /.
we all are DIY'ers here.</sentencetext>
	<parent>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30907066</parent>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30908706</id>
	<title>or Frank Herbert's take on DIY, at-home biology</title>
	<author>Anonymous</author>
	<datestamp>1264538400000</datestamp>
	<modclass>Informativ</modclass>
	<modscore>1</modscore>
	<htmltext><p><a href="http://en.wikipedia.org/wiki/The\_White\_Plague" title="wikipedia.org" rel="nofollow">http://en.wikipedia.org/wiki/The\_White\_Plague</a> [wikipedia.org]</p><p>Scared me way more than any "grey goo" idea.</p></htmltext>
<tokenext>http : //en.wikipedia.org/wiki/The \ _White \ _Plague [ wikipedia.org ] Scared me way more than any " grey goo " idea .</tokentext>
<sentencetext>http://en.wikipedia.org/wiki/The\_White\_Plague [wikipedia.org]Scared me way more than any "grey goo" idea.</sentencetext>
	<parent>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30906996</parent>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30910960</id>
	<title>Re:Depends</title>
	<author>interkin3tic</author>
	<datestamp>1264505040000</datestamp>
	<modclass>Informativ</modclass>
	<modscore>2</modscore>
	<htmltext><p><div class="quote"><p>Many of these biology experiments require very expensive machines, such as microarray machines, as mentioned by the article. I don't know if purchasing refurbished machines is a wise choice since we don't want data quality to be compromised.</p> </div><p>A microarray is pretty expensive yes, but a lot of DIY biology could be done with just a computer and or a secondhand PCR machine.  Used PCR machines <a href="http://shop.ebay.com/?\_from=R40&amp;\_trksid=p3907.m38.l1313&amp;\_nkw=PCR&amp;\_sacat=See-All-Categories" title="ebay.com"> apperantly can be had for under a grand</a> [ebay.com].  Even less if you can service a broken one yourself, which many of these DIYers seem capable of.  Probably won't have all the fancy options of a higher priced one either, but our academic lab has an expensive cycler with many options that we never use.</p><p>Data quality with many of these things is less tempermental than a microarray too.  The secondhand PCR machine in this case might not be good for sequencing, but it would be a great tool if you were, say, making a plasmid to make glowing bacteria, using it to identify species of plants, making in-situ hybridization primers.  There are a lot of things you can do with a basic cheap PCR machine.</p><p>As far as microarray data goes, an affymetrix premade microarray chip goes for about a thousand dollars.  Obviously it's not feasible for most people to do many of these out of their own pocket, but not everyone does.  Say you want to find out what genes are expressed more in dog breed A than dog breed B.  If you were wanting to publish that data in a peer-reviewed journal, you'd probably need 6 chips, it seems like most people I know who do microarray do triplicates.  If you were just wanting to find out for yourself, like to find canidates for which genes produced trait X that was in breed A, you could do just two, one for each, and hope it wasn't wildly innacurate.  You could then focus your search based on that, taking it with a grain of salt until you confirmed it through other, less expensive means.</p><p>If you were going to be doing many microarrays, <a href="http://cmgm.stanford.edu/pbrown/mguide/" title="stanford.edu">this website</a> [stanford.edu] appears to be a guide for making your own microarrayer.  The price tag for building it exactly as that lab says to would be <a href="http://cmgm.stanford.edu/pbrown/mguide/partslist.html" title="stanford.edu">about $24k</a> [stanford.edu].  Again though, many DIYers are mechanically inclined and could cut corners for their own purposes.</p><p><div class="quote"><p>Another issue is gathering the samples. If you're collecting yeast, that would be simple. Arabidopsis, other small plants, mice, or other small animals, you probably need quite some space.</p></div><p>I don't see that.  Our lab studies chicken embryos.  An egg incubator is pretty small.  C elegans can be grown wherever you've got space.  Arabidopsis can grow in the yard, you don't need acres.  A research-grade mouse colony would be expensive yes (maintaining a genetically pure mouse colony in a sterile environment free of variation is harder just obtaining mice from the street).  If you need other model organisms, there are farms.  It can be a limiting factor, yes, but when is that not true?  You can't exactly use elephants as a model organism in really any lab in the world.</p><p><div class="quote"><p>Humans? That won't be simple at all. You have to clear privacy issues, getting the research review board to sign papers, etc.</p></div><p>Which research review board?  If I'm comparing gene expression in human blood samples in my garage, without using public grant money, the "review board" is whatever poor saps I sucker into giving me their blood.</p><p><div class="quote"><p> You can always resort to publicly available data. But chances are that you won't be able to impress scientists much for going that route.  Also, most of the important discoveries are already done on this data.</p> </div><p>I reject both of those claims.  Real scientists recognize valid results independant of the professional nature of the researcher or his lab.  Hell, most of us "professionals" are pretty much loony toons anyway, some nut doing experiments in his garage is just as believable as some nut getting paid to do it.  Journal editors might not be, but many won't.</p><p>As far as "all that's worth knowing from public data sets is already known," that's wayyyy off.  The specific area of your specific field you're thinking of... maybe, but I doubt it.  Across all publicly available data?  Just no.</p><p>For example, genomes are being published all the time.  I'd submit that a genome just published a few months ago cannot possibly be tapped out.  Find some homologous genes in far-diverged species, like sea urchin or platypus, and do the analysis from your own computer on your own time, using the free resources out there.  There are plenty of interesting and useful discoveries left there on important genes.</p><p><div class="quote"><p>Most likely, all you can do is to confirm existing results or to provide some tangential additional info.</p></div><p>Confirming existing results is a necessary and valuable task in science that, by and large, is not being done by biological labs thanks to the publish-or-perish system and the requirement by most journals that results be new and fresh.</p><p>"Tangential" additional info in biology is rarely useless and is also often neglected by professional labs for much the same reason as confirming results.</p><p>
&nbsp;</p></div>
	</htmltext>
<tokenext>Many of these biology experiments require very expensive machines , such as microarray machines , as mentioned by the article .
I do n't know if purchasing refurbished machines is a wise choice since we do n't want data quality to be compromised .
A microarray is pretty expensive yes , but a lot of DIY biology could be done with just a computer and or a secondhand PCR machine .
Used PCR machines apperantly can be had for under a grand [ ebay.com ] .
Even less if you can service a broken one yourself , which many of these DIYers seem capable of .
Probably wo n't have all the fancy options of a higher priced one either , but our academic lab has an expensive cycler with many options that we never use.Data quality with many of these things is less tempermental than a microarray too .
The secondhand PCR machine in this case might not be good for sequencing , but it would be a great tool if you were , say , making a plasmid to make glowing bacteria , using it to identify species of plants , making in-situ hybridization primers .
There are a lot of things you can do with a basic cheap PCR machine.As far as microarray data goes , an affymetrix premade microarray chip goes for about a thousand dollars .
Obviously it 's not feasible for most people to do many of these out of their own pocket , but not everyone does .
Say you want to find out what genes are expressed more in dog breed A than dog breed B. If you were wanting to publish that data in a peer-reviewed journal , you 'd probably need 6 chips , it seems like most people I know who do microarray do triplicates .
If you were just wanting to find out for yourself , like to find canidates for which genes produced trait X that was in breed A , you could do just two , one for each , and hope it was n't wildly innacurate .
You could then focus your search based on that , taking it with a grain of salt until you confirmed it through other , less expensive means.If you were going to be doing many microarrays , this website [ stanford.edu ] appears to be a guide for making your own microarrayer .
The price tag for building it exactly as that lab says to would be about $ 24k [ stanford.edu ] .
Again though , many DIYers are mechanically inclined and could cut corners for their own purposes.Another issue is gathering the samples .
If you 're collecting yeast , that would be simple .
Arabidopsis , other small plants , mice , or other small animals , you probably need quite some space.I do n't see that .
Our lab studies chicken embryos .
An egg incubator is pretty small .
C elegans can be grown wherever you 've got space .
Arabidopsis can grow in the yard , you do n't need acres .
A research-grade mouse colony would be expensive yes ( maintaining a genetically pure mouse colony in a sterile environment free of variation is harder just obtaining mice from the street ) .
If you need other model organisms , there are farms .
It can be a limiting factor , yes , but when is that not true ?
You ca n't exactly use elephants as a model organism in really any lab in the world.Humans ?
That wo n't be simple at all .
You have to clear privacy issues , getting the research review board to sign papers , etc.Which research review board ?
If I 'm comparing gene expression in human blood samples in my garage , without using public grant money , the " review board " is whatever poor saps I sucker into giving me their blood .
You can always resort to publicly available data .
But chances are that you wo n't be able to impress scientists much for going that route .
Also , most of the important discoveries are already done on this data .
I reject both of those claims .
Real scientists recognize valid results independant of the professional nature of the researcher or his lab .
Hell , most of us " professionals " are pretty much loony toons anyway , some nut doing experiments in his garage is just as believable as some nut getting paid to do it .
Journal editors might not be , but many wo n't.As far as " all that 's worth knowing from public data sets is already known , " that 's wayyyy off .
The specific area of your specific field you 're thinking of... maybe , but I doubt it .
Across all publicly available data ?
Just no.For example , genomes are being published all the time .
I 'd submit that a genome just published a few months ago can not possibly be tapped out .
Find some homologous genes in far-diverged species , like sea urchin or platypus , and do the analysis from your own computer on your own time , using the free resources out there .
There are plenty of interesting and useful discoveries left there on important genes.Most likely , all you can do is to confirm existing results or to provide some tangential additional info.Confirming existing results is a necessary and valuable task in science that , by and large , is not being done by biological labs thanks to the publish-or-perish system and the requirement by most journals that results be new and fresh .
" Tangential " additional info in biology is rarely useless and is also often neglected by professional labs for much the same reason as confirming results .
 </tokentext>
<sentencetext>Many of these biology experiments require very expensive machines, such as microarray machines, as mentioned by the article.
I don't know if purchasing refurbished machines is a wise choice since we don't want data quality to be compromised.
A microarray is pretty expensive yes, but a lot of DIY biology could be done with just a computer and or a secondhand PCR machine.
Used PCR machines  apperantly can be had for under a grand [ebay.com].
Even less if you can service a broken one yourself, which many of these DIYers seem capable of.
Probably won't have all the fancy options of a higher priced one either, but our academic lab has an expensive cycler with many options that we never use.Data quality with many of these things is less tempermental than a microarray too.
The secondhand PCR machine in this case might not be good for sequencing, but it would be a great tool if you were, say, making a plasmid to make glowing bacteria, using it to identify species of plants, making in-situ hybridization primers.
There are a lot of things you can do with a basic cheap PCR machine.As far as microarray data goes, an affymetrix premade microarray chip goes for about a thousand dollars.
Obviously it's not feasible for most people to do many of these out of their own pocket, but not everyone does.
Say you want to find out what genes are expressed more in dog breed A than dog breed B.  If you were wanting to publish that data in a peer-reviewed journal, you'd probably need 6 chips, it seems like most people I know who do microarray do triplicates.
If you were just wanting to find out for yourself, like to find canidates for which genes produced trait X that was in breed A, you could do just two, one for each, and hope it wasn't wildly innacurate.
You could then focus your search based on that, taking it with a grain of salt until you confirmed it through other, less expensive means.If you were going to be doing many microarrays, this website [stanford.edu] appears to be a guide for making your own microarrayer.
The price tag for building it exactly as that lab says to would be about $24k [stanford.edu].
Again though, many DIYers are mechanically inclined and could cut corners for their own purposes.Another issue is gathering the samples.
If you're collecting yeast, that would be simple.
Arabidopsis, other small plants, mice, or other small animals, you probably need quite some space.I don't see that.
Our lab studies chicken embryos.
An egg incubator is pretty small.
C elegans can be grown wherever you've got space.
Arabidopsis can grow in the yard, you don't need acres.
A research-grade mouse colony would be expensive yes (maintaining a genetically pure mouse colony in a sterile environment free of variation is harder just obtaining mice from the street).
If you need other model organisms, there are farms.
It can be a limiting factor, yes, but when is that not true?
You can't exactly use elephants as a model organism in really any lab in the world.Humans?
That won't be simple at all.
You have to clear privacy issues, getting the research review board to sign papers, etc.Which research review board?
If I'm comparing gene expression in human blood samples in my garage, without using public grant money, the "review board" is whatever poor saps I sucker into giving me their blood.
You can always resort to publicly available data.
But chances are that you won't be able to impress scientists much for going that route.
Also, most of the important discoveries are already done on this data.
I reject both of those claims.
Real scientists recognize valid results independant of the professional nature of the researcher or his lab.
Hell, most of us "professionals" are pretty much loony toons anyway, some nut doing experiments in his garage is just as believable as some nut getting paid to do it.
Journal editors might not be, but many won't.As far as "all that's worth knowing from public data sets is already known," that's wayyyy off.
The specific area of your specific field you're thinking of... maybe, but I doubt it.
Across all publicly available data?
Just no.For example, genomes are being published all the time.
I'd submit that a genome just published a few months ago cannot possibly be tapped out.
Find some homologous genes in far-diverged species, like sea urchin or platypus, and do the analysis from your own computer on your own time, using the free resources out there.
There are plenty of interesting and useful discoveries left there on important genes.Most likely, all you can do is to confirm existing results or to provide some tangential additional info.Confirming existing results is a necessary and valuable task in science that, by and large, is not being done by biological labs thanks to the publish-or-perish system and the requirement by most journals that results be new and fresh.
"Tangential" additional info in biology is rarely useless and is also often neglected by professional labs for much the same reason as confirming results.
 
	</sentencetext>
	<parent>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30907022</parent>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30908982</id>
	<title>Re:Depends</title>
	<author>Maglos</author>
	<datestamp>1264496460000</datestamp>
	<modclass>None</modclass>
	<modscore>1</modscore>
	<htmltext>Some guys will spend 100+k on a wood working shop; I'm sure there are enough people with money to put a few dollars down on cool biotech toys. A professor of mine bought a 30k drafting printer for printing large format photography.</htmltext>
<tokenext>Some guys will spend 100 + k on a wood working shop ; I 'm sure there are enough people with money to put a few dollars down on cool biotech toys .
A professor of mine bought a 30k drafting printer for printing large format photography .</tokentext>
<sentencetext>Some guys will spend 100+k on a wood working shop; I'm sure there are enough people with money to put a few dollars down on cool biotech toys.
A professor of mine bought a 30k drafting printer for printing large format photography.</sentencetext>
	<parent>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30907022</parent>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30907066</id>
	<title>DIY??</title>
	<author>Anonymous</author>
	<datestamp>1264531620000</datestamp>
	<modclass>Funny</modclass>
	<modscore>4</modscore>
	<htmltext><p>Do it <b>yourself </b> biology??</p><p>I prefer "do it with someone else" biology...</p></htmltext>
<tokenext>Do it yourself biology ?
? I prefer " do it with someone else " biology.. .</tokentext>
<sentencetext>Do it yourself  biology?
?I prefer "do it with someone else" biology...</sentencetext>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30907866</id>
	<title>Re:Uhhh...</title>
	<author>mcgrew</author>
	<datestamp>1264535040000</datestamp>
	<modclass>None</modclass>
	<modscore>1</modscore>
	<htmltext><p>Naw, it's easy. The hard part is finding a lab partner. If it wasn't for DIY biology I wouldn't have kids.</p></htmltext>
<tokenext>Naw , it 's easy .
The hard part is finding a lab partner .
If it was n't for DIY biology I would n't have kids .</tokentext>
<sentencetext>Naw, it's easy.
The hard part is finding a lab partner.
If it wasn't for DIY biology I wouldn't have kids.</sentencetext>
	<parent>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30906858</parent>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30912876</id>
	<title>Re:Kidding, right?</title>
	<author>interkin3tic</author>
	<datestamp>1264519140000</datestamp>
	<modclass>None</modclass>
	<modscore>1</modscore>
	<htmltext><p><div class="quote"><p>Discarding ethidium-bromide and acrylamide gels?</p></div><p>If I were doing DNA gels at home, I'd be using <a href="http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Nucleic-Acid-Purification-and-Analysis/Nucleic-Acid-Gel-Electrophoresis/nucleic\_acid\_gel\_electrophoresis/Nucleic-Acid-Stains/SybrSafe-A-better-DNA-gel-stain.html" title="invitrogen.com">sybrsafe</a> [invitrogen.com].  As the name implies, it's a lot safer.  Also less damaging to the DNA I'd be trying to isolate and can be reused many times.  Slightly more expensive than dirt-cheap ethidium bromide though.</p><p>While unpolymerized acrylamide is a pretty dangerous neurotoxin, a polymerized polyacrylamide gel should actually be pretty safe.  Granted, I wouldn't trust it, but plenty of academic labs do throw their in the garbage.</p><p><div class="quote"><p>Biological experiments are different from software development, they need follow up and supervision through the end, which may take 2-4 days.</p></div><p>Some do, yes, but I'd say that doesn't describe most biological experiments.  There are times when I need to work for half a day without interruption, but those are rare.</p><p><div class="quote"><p>How do you discard biohazardous materials and mutagen/teratogen substances at home?</p></div><p>Depends on the hazardous/mutagenic material.  There are quite detailed protocols and guidelines for disposal of almost all waste coming out of labs, with a little thinking you can find facilities to dispose of it, or find a way to neurtralize it yourself.  And it's not like all research requires working with plutonium or exotic and dangerous materials.  The most common teratogen I'd probably pour down the sink or drink (the most common teratogen being alchohol.)</p></div>
	</htmltext>
<tokenext>Discarding ethidium-bromide and acrylamide gels ? If I were doing DNA gels at home , I 'd be using sybrsafe [ invitrogen.com ] .
As the name implies , it 's a lot safer .
Also less damaging to the DNA I 'd be trying to isolate and can be reused many times .
Slightly more expensive than dirt-cheap ethidium bromide though.While unpolymerized acrylamide is a pretty dangerous neurotoxin , a polymerized polyacrylamide gel should actually be pretty safe .
Granted , I would n't trust it , but plenty of academic labs do throw their in the garbage.Biological experiments are different from software development , they need follow up and supervision through the end , which may take 2-4 days.Some do , yes , but I 'd say that does n't describe most biological experiments .
There are times when I need to work for half a day without interruption , but those are rare.How do you discard biohazardous materials and mutagen/teratogen substances at home ? Depends on the hazardous/mutagenic material .
There are quite detailed protocols and guidelines for disposal of almost all waste coming out of labs , with a little thinking you can find facilities to dispose of it , or find a way to neurtralize it yourself .
And it 's not like all research requires working with plutonium or exotic and dangerous materials .
The most common teratogen I 'd probably pour down the sink or drink ( the most common teratogen being alchohol .
)</tokentext>
<sentencetext>Discarding ethidium-bromide and acrylamide gels?If I were doing DNA gels at home, I'd be using sybrsafe [invitrogen.com].
As the name implies, it's a lot safer.
Also less damaging to the DNA I'd be trying to isolate and can be reused many times.
Slightly more expensive than dirt-cheap ethidium bromide though.While unpolymerized acrylamide is a pretty dangerous neurotoxin, a polymerized polyacrylamide gel should actually be pretty safe.
Granted, I wouldn't trust it, but plenty of academic labs do throw their in the garbage.Biological experiments are different from software development, they need follow up and supervision through the end, which may take 2-4 days.Some do, yes, but I'd say that doesn't describe most biological experiments.
There are times when I need to work for half a day without interruption, but those are rare.How do you discard biohazardous materials and mutagen/teratogen substances at home?Depends on the hazardous/mutagenic material.
There are quite detailed protocols and guidelines for disposal of almost all waste coming out of labs, with a little thinking you can find facilities to dispose of it, or find a way to neurtralize it yourself.
And it's not like all research requires working with plutonium or exotic and dangerous materials.
The most common teratogen I'd probably pour down the sink or drink (the most common teratogen being alchohol.
)
	</sentencetext>
	<parent>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30907610</parent>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30907182</id>
	<title>Re:Uhhh...</title>
	<author>izomiac</author>
	<datestamp>1264532100000</datestamp>
	<modclass>Insightful</modclass>
	<modscore>2</modscore>
	<htmltext>Meh, most lab research in biology fail on the first few attempts.  DIY biology is likely to have an even higher failure rate, especially with stuff that hasn't been done a thousand times before.  Beyond that, if grey goo or a super bug was feasible the natural bacteria would've done it ages ago.</htmltext>
<tokenext>Meh , most lab research in biology fail on the first few attempts .
DIY biology is likely to have an even higher failure rate , especially with stuff that has n't been done a thousand times before .
Beyond that , if grey goo or a super bug was feasible the natural bacteria would 've done it ages ago .</tokentext>
<sentencetext>Meh, most lab research in biology fail on the first few attempts.
DIY biology is likely to have an even higher failure rate, especially with stuff that hasn't been done a thousand times before.
Beyond that, if grey goo or a super bug was feasible the natural bacteria would've done it ages ago.</sentencetext>
	<parent>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30906858</parent>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30907022</id>
	<title>Depends</title>
	<author>Anonymous</author>
	<datestamp>1264531500000</datestamp>
	<modclass>Insightful</modclass>
	<modscore>2</modscore>
	<htmltext><p>Many of these biology experiments require very expensive machines, such as microarray machines, as mentioned by the article. I don't know if purchasing refurbished machines is a wise choice since we don't want data quality to be compromised. Also, don't forget about service plans when the machines break or producing inconsistent output. Not to mention various reagents, other chemicals, and supplies such as microarray chips that make the experiment yields high quality data. These easily reach hundreds of dollars a piece. Also, purchasing such chemicals will get you labeled as a terrorist.</p><p>Another issue is gathering the samples. If you're collecting yeast, that would be simple. Arabidopsis, other small plants, mice, or other small animals, you probably need quite some space. Humans? That won't be simple at all. You have to clear privacy issues, getting the research review board to sign papers, etc. Sample collection alone can cost you lots of money and time. You can always resort to publicly available data. But chances are that you won't be able to impress scientists much for going that route. Also, most of the important discoveries are already done on this data. Most likely, all you can do is to confirm existing results or to provide some tangential additional info.</p></htmltext>
<tokenext>Many of these biology experiments require very expensive machines , such as microarray machines , as mentioned by the article .
I do n't know if purchasing refurbished machines is a wise choice since we do n't want data quality to be compromised .
Also , do n't forget about service plans when the machines break or producing inconsistent output .
Not to mention various reagents , other chemicals , and supplies such as microarray chips that make the experiment yields high quality data .
These easily reach hundreds of dollars a piece .
Also , purchasing such chemicals will get you labeled as a terrorist.Another issue is gathering the samples .
If you 're collecting yeast , that would be simple .
Arabidopsis , other small plants , mice , or other small animals , you probably need quite some space .
Humans ? That wo n't be simple at all .
You have to clear privacy issues , getting the research review board to sign papers , etc .
Sample collection alone can cost you lots of money and time .
You can always resort to publicly available data .
But chances are that you wo n't be able to impress scientists much for going that route .
Also , most of the important discoveries are already done on this data .
Most likely , all you can do is to confirm existing results or to provide some tangential additional info .</tokentext>
<sentencetext>Many of these biology experiments require very expensive machines, such as microarray machines, as mentioned by the article.
I don't know if purchasing refurbished machines is a wise choice since we don't want data quality to be compromised.
Also, don't forget about service plans when the machines break or producing inconsistent output.
Not to mention various reagents, other chemicals, and supplies such as microarray chips that make the experiment yields high quality data.
These easily reach hundreds of dollars a piece.
Also, purchasing such chemicals will get you labeled as a terrorist.Another issue is gathering the samples.
If you're collecting yeast, that would be simple.
Arabidopsis, other small plants, mice, or other small animals, you probably need quite some space.
Humans? That won't be simple at all.
You have to clear privacy issues, getting the research review board to sign papers, etc.
Sample collection alone can cost you lots of money and time.
You can always resort to publicly available data.
But chances are that you won't be able to impress scientists much for going that route.
Also, most of the important discoveries are already done on this data.
Most likely, all you can do is to confirm existing results or to provide some tangential additional info.</sentencetext>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30911012</id>
	<title>Re:Uhhh...</title>
	<author>Eudial</author>
	<datestamp>1264505340000</datestamp>
	<modclass>None</modclass>
	<modscore>1</modscore>
	<htmltext><p><div class="quote"><p>DIY Biology sounds pretty dangerous.</p><p>As long as the instructions it comes with are better than Ikea's...</p></div><p>Not particularly so long as you're careful and use a condom.</p></div>
	</htmltext>
<tokenext>DIY Biology sounds pretty dangerous.As long as the instructions it comes with are better than Ikea 's...Not particularly so long as you 're careful and use a condom .</tokentext>
<sentencetext>DIY Biology sounds pretty dangerous.As long as the instructions it comes with are better than Ikea's...Not particularly so long as you're careful and use a condom.
	</sentencetext>
	<parent>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30906858</parent>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30906934</id>
	<title>Distributed Cluster</title>
	<author>Anonymous</author>
	<datestamp>1264531140000</datestamp>
	<modclass>Funny</modclass>
	<modscore>0</modscore>
	<htmltext><p><nobr> <wbr></nobr></p><div class="quote"><p>...Folding@home, is now the most powerful distributed computing cluster in the world...</p></div><p>A Distributed Cluster?  *Head Asplode*</p></div>
	</htmltext>
<tokenext>...Folding @ home , is now the most powerful distributed computing cluster in the world...A Distributed Cluster ?
* Head Asplode *</tokentext>
<sentencetext> ...Folding@home, is now the most powerful distributed computing cluster in the world...A Distributed Cluster?
*Head Asplode*
	</sentencetext>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30906894</id>
	<title>Re:Uhhh...</title>
	<author>Anonymous</author>
	<datestamp>1264530960000</datestamp>
	<modclass>Offtopic</modclass>
	<modscore>-1</modscore>
	<htmltext><p>All civilians must be locked in dark, padded rooms until they have been trained for their state selected profession. Amirite?</p></htmltext>
<tokenext>All civilians must be locked in dark , padded rooms until they have been trained for their state selected profession .
Amirite ?</tokentext>
<sentencetext>All civilians must be locked in dark, padded rooms until they have been trained for their state selected profession.
Amirite?</sentencetext>
	<parent>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30906858</parent>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30912124</id>
	<title>The open source flu</title>
	<author>geekoid</author>
	<datestamp>1264512540000</datestamp>
	<modclass>Funny</modclass>
	<modscore>2</modscore>
	<htmltext><p>coming to a upper respiratory near you.</p></htmltext>
<tokenext>coming to a upper respiratory near you .</tokentext>
<sentencetext>coming to a upper respiratory near you.</sentencetext>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30907098</id>
	<title>Is this really a new thing?</title>
	<author>Locke2005</author>
	<datestamp>1264531800000</datestamp>
	<modclass>Redundant</modclass>
	<modscore>0</modscore>
	<htmltext>People have been experimenting with DIY biology for years. My first experiment is named "Venus", she is 9 years old now and is a sweet, lovable (if hyperactive) cheerleader. Overall, I'd say this experiment was a resounding success, although I am still waiting for others to replicate my results.</htmltext>
<tokenext>People have been experimenting with DIY biology for years .
My first experiment is named " Venus " , she is 9 years old now and is a sweet , lovable ( if hyperactive ) cheerleader .
Overall , I 'd say this experiment was a resounding success , although I am still waiting for others to replicate my results .</tokentext>
<sentencetext>People have been experimenting with DIY biology for years.
My first experiment is named "Venus", she is 9 years old now and is a sweet, lovable (if hyperactive) cheerleader.
Overall, I'd say this experiment was a resounding success, although I am still waiting for others to replicate my results.</sentencetext>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30907176</id>
	<title>Dammit</title>
	<author>JustNiz</author>
	<datestamp>1264532100000</datestamp>
	<modclass>Funny</modclass>
	<modscore>2</modscore>
	<htmltext><p>From the title I was looking forward to the news that I could DL the opensource software, get my PC hooked up to a robot arm and a webcam,  and have it do my appendectomy.</p></htmltext>
<tokenext>From the title I was looking forward to the news that I could DL the opensource software , get my PC hooked up to a robot arm and a webcam , and have it do my appendectomy .</tokentext>
<sentencetext>From the title I was looking forward to the news that I could DL the opensource software, get my PC hooked up to a robot arm and a webcam,  and have it do my appendectomy.</sentencetext>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30923626</id>
	<title>Re:Any progress?</title>
	<author>badkarmadayaccount</author>
	<datestamp>1264584120000</datestamp>
	<modclass>None</modclass>
	<modscore>1</modscore>
	<htmltext><p><div class="quote"><p>&lt;snip&gt;(and species!)&lt;/snip&gt;</p></div><p>Only on Slashdot.</p></div>
	</htmltext>
<tokenext>( and species !
) Only on Slashdot .</tokentext>
<sentencetext>(and species!
)Only on Slashdot.
	</sentencetext>
	<parent>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30907092</parent>
</comment>
<comment>
	<id>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30906924</id>
	<title>Re:Uhhh...</title>
	<author>Anonymous</author>
	<datestamp>1264531080000</datestamp>
	<modclass>Troll</modclass>
	<modscore>-1</modscore>
	<htmltext><p>You represent the pussification of western society.<br>Well done, and GTFO</p></htmltext>
<tokenext>You represent the pussification of western society.Well done , and GTFO</tokentext>
<sentencetext>You represent the pussification of western society.Well done, and GTFO</sentencetext>
	<parent>http://www.semanticweb.org/ontologies/ConversationInstances.owl#comment10_01_26_165254.30906858</parent>
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